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Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis

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Summary

The separation of derivatized carbohydrates has been performed by co-electroosmotic capillary electrophoresis. Derivatization was performed by reductive amination of the carbohydrates with ethylp-aminobenzoate or withp-aminobenzonitrile. Separation selectivity is optimized using buffer electrolytes containing high concentrations of borate, organic solvents, and mixtures thereof; this enabled separation of the carbohydrate derivatives then direct UV detection. Co-directional migration of the anionic analytes with the electroosmotic flow was achieved by adding a cationic polyer (hexadimethrine bromide, HDB) to the electrolyte. With this method it is possible to determine specific carbohydrates, such as arabinose, mannose, and glucose, which are difficult to separate by other CE methods. The applicability of the method is demonstrated for the analysis of plant hydrolyzates

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Authors and Affiliations

  1. Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens-University Innsbruck, Innrain 52a, 6020, Innsbruck, Austria

    D. T. Nguyen, H. Lerch, A. Zemann & G. Bonn

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  1. D. T. Nguyen
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  2. H. Lerch
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  3. A. Zemann
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  4. G. Bonn
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Nguyen, D.T., Lerch, H., Zemann, A. et al. Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis. Chromatographia 46, 113–121 (1997). https://doi.org/10.1007/BF02495320

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  • DOI: https://doi.org/10.1007/BF02495320

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