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Selective solid phase extraction of a drug lead compound using molecularly imprinted polymers prepared by the target analogue approach

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  • Solid Phase Extraction
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Summary

Molecularly imprinted polymers have been evaluated at the sample clean-up stage in the analysis of a drug lead compound. In order to circumvent quantification problems related to bleeding of the template, a structurally related analogue of the latter was used. This was selected based on criteria related to interaction site location, solubility, availability and stability of the analogue. Selection of suitable polymerisation conditions was then made using a small batch format (ca. 50 mg) and rapid assessment of binding in the equilibrium mode. It was found that the amount of template could be greatly reduced compared to the conventional protocol, requiring only 5 μmol of template per gram of polymer without seriously compromising the performance of the materials for chromatographic or SPE applications.

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Authors and Affiliations

  1. Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg Universität Mainz, Duesbergweg 10-14, 55099, Mainz, Germany

    B. Dirion, F. Lanza & B. Sellergren

  2. Bioanalytical Group, Department of Drug Metabolism, Pfizer Global Research and Development, CT13 9NJ, Sandwich, Kent, UK

    C. Chassaing & R. Venn

  3. MIP Technologies AB, Research Park Ideon, 22370, Lund, Sweden

    C. Berggren

Authors
  1. B. Dirion
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  2. F. Lanza
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  3. B. Sellergren
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  4. C. Chassaing
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  5. R. Venn
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  6. C. Berggren
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Dirion, B., Lanza, F., Sellergren, B. et al. Selective solid phase extraction of a drug lead compound using molecularly imprinted polymers prepared by the target analogue approach. Chromatographia 56, 237–241 (2002). https://doi.org/10.1007/BF02493219

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  • DOI: https://doi.org/10.1007/BF02493219

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