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Use of a database of plots of pesticide retention (R F) against mobile-phase composition.Part II. TLC as a pilot technique for transferring retention data to HPLC, and use of the data for preliminary fractionation of a mixture of pesticides by micropreparative column chromatography

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Summary

Relationships betweenR F values and mobile-phase composition have been determined for moderately polar pesticides in normal-phase systems (NP) of the type silica-non-polar diluent (heptane)-polar modifier (ethyl acetate, tetrahydrofuran, or dioxane) and in reversed-phase systems (RP) of the type octadecyl silica-water-polar modifier (acetonitrile, methanol, or tetrahydrofuran). These relationships constitute a retention database which has enabled choice of the optimum conditions for preparative column chromatographic separation of pesticides into fractions; these were then applied to a silica plate and chromatographed. The plate was videoscanned, furnishing a real picture of the plate showing complete separation of the pesticide fractions.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Medical University, Staszica 6, 20-081, Lublin, Poland

    T. Tuzimski & E. Soczewinski

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  1. T. Tuzimski
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  2. E. Soczewinski
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Tuzimski, T., Soczewinski, E. Use of a database of plots of pesticide retention (R F) against mobile-phase composition.Part II. TLC as a pilot technique for transferring retention data to HPLC, and use of the data for preliminary fractionation of a mixture of pesticides by micropreparative column chromatography. Chromatographia 56, 225–227 (2002). https://doi.org/10.1007/BF02493216

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  • DOI: https://doi.org/10.1007/BF02493216

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