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A novel silica-based column packing for reversed-phase HPLC of basic compounds

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Summary

A novel bonded phase for reversed-phase HPLC was synthesized in two steps. Octylamine was first reacted with β-(3,4-epoxycyclohexyl)ethyltrimethoxysilane (β-ECTS) and then the intermediate product was coupled onto porous silica. The prepared packing was characterized by elemental analysis, solid-state13C NMR and Fourier transform infrared (FT-IR). Chromatographic evaluations were carried out by using a mixture of organic compounds including acidic, basic and neutral analytes and methanol-water as binary mobile phase. The results showed that the stationary phase has excellent chromatographic properties and is resistant to hydrolysis between pH=2≈8. It can be used efficiently for the separation of basic compounds.

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References

  1. Major, R.E.LC-GC Mag 1991,9, 686–693.

    Google Scholar 

  2. Major, R.E.LC-GC 1998,16(3), 228–244.

    Google Scholar 

  3. Hanai, T.; Ohhira, M.; Tamura, T.LC-GC 1988,6, 922–928.

    CAS  Google Scholar 

  4. Freiser, H.H.; Nowlan, M.P.; Gooding, D.L.J. Liq. Chromatogr. 1989,12, 827–843.

    CAS  Google Scholar 

  5. Ohstu, Y.; Shiojima, Y.; Okumura, T.; Koyama, J.I.; et al.J. Chromatogr. 1989 481, 147–157.

    Article  Google Scholar 

  6. Stadalius, M.A.; Berus, J.S.; Synder, L.R.LC-GC 1988,6, 494–500.

    CAS  Google Scholar 

  7. O'Gara, J.E.; Alden, B.A.; Walter, T.H.; Petersen, J.S.; et al.Anal. Chem. 1995,67, 3809–3813.

    Article  Google Scholar 

  8. Kirkland, J.J.; Henderson, J.W.J. Chromatogr. Sci. 1994,32, 473–478.

    CAS  Google Scholar 

  9. Kirkland, J.J.; Henderson, J.W.; Martosella, J.D.; Bidlingmeyer, B.A.; Basta-Russell, J.; Adams, Jr, J.B.LC-GC. 1999,17(7), 634–639.

    CAS  Google Scholar 

  10. Xin, Y.; Rui, Z.; Guoquan, L.J. Liq. Chromatogr. & Rel. Technol. 2000 23 (12), 1821–1830.

    Article  Google Scholar 

  11. Bayer, E.; Albert, J.; Reiners, J.; Nieder, M.J. Chromatogr. 1983,264, 197–213.

    Article  CAS  Google Scholar 

  12. Engelhard, H.; Arangio, M.; Lobert, H.LC-GC 1997,15 (9), 856–864.

    Google Scholar 

  13. Engelhard, H.; Jungheim, M.Chromatographia 1990,29 (1/2), 59–68.

    Article  Google Scholar 

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Authors and Affiliations

  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, 116012, Dalian, People's Republic of China

    Xiaojia Huang, Junde Wang, Xueliang Liu & Zhenhua Shang

Authors
  1. Xiaojia Huang
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  2. Junde Wang
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  3. Xueliang Liu
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  4. Zhenhua Shang
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Huang, X., Wang, J., Liu, X. et al. A novel silica-based column packing for reversed-phase HPLC of basic compounds. Chromatographia 54, 350–354 (2001). https://doi.org/10.1007/BF02492682

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  • DOI: https://doi.org/10.1007/BF02492682

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