Summary
Medroxyprogesterone acetate (MPA) is one of the major drugs used in endocrine therapy for advanced or recurrent breast cancer. The present study describes an accurate and reproductible gas chromatographic-mass spectrometric(GC-MS) method to measure MPA in serum.
After being extracted by a solid-phase cartridge using an Oasis HLB copolymer, the residues were derivatized with a heptafluorobutyric anhydride and then analyzed by GC-MS.
We validated the method by analyzing the serum samples spiked with pure MPA, and obtained a linear correlation (0.9977) and precision with a coefficient of variation ranging from 1.8 to 7.3. We found that the GC-MS method was linear over the range of 0.2 ng mL−1 to 500 ng mL−1 using a sample volume of 1 mL. The analytical recovery was 93.7%, and the limit of detection was 0.2 ng mL−1.
The oral administration of MPA to men and its subsequent detection in serum showed that the GC-MS method described is sensitive, specific, and suitable for the determination of MPA by bench-top GC-MS.
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Choi, M.H., Kim, K.R., Kim, D.H. et al. Rapid and sensitive analysis of medroxyprogesterone acetate in human serum by gas chromatography-mass spectrometry. Chromatographia 54, 765–769 (2001). https://doi.org/10.1007/BF02492496
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DOI: https://doi.org/10.1007/BF02492496