Skip to main content
SpringerLink
Log in

An LC/MS/MS method for quantifying testosterone and dehydroepiandrosterone sulfate in four different serum samples during a single run

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Simultaneous measurements of the circulating testosterone (TS) and dehydroepiandrosterone sulfate (DHEAS) are deemed to be helpful for the assessment of men’s health. Liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI–MS/MS) is the most reliable methodology for this purpose; however, it has room for improvement in analysis throughput. In this study, a quadruplicate of the Girard reagents was used to develop an LC/ESI–MS/MS method capable of quantifying TS and DHEAS in four different serum samples in a single run. The four serum samples were separately pretreated, derivatized with one of four Girard reagents, and then combined. The LC/ESI–MS/MS analysis of the combined sample provided the androgen concentrations of four serum samples in parallel. The method had practical measuring ranges, in which good precision and accuracy, as well as negligible matrix effects were verified. The speed-up capability of the developed method was evaluated through the analysis of ten batches of serum samples (total 40 samples); the method saved a 60% post-pretreatment analysis time compared to the non-derivatization method for 40 samples.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. M.W. O’Reilly, M. Glisic, B. Kumarendran, A. Subramanian, K.N. Manolopoulos, A.A. Tahrani, D. Keerthy, T. Muka, K.A. Toulis, W. Hanif, G.N. Thomas, O.H. Franco, W. Arlt, K. Nirantharakumar, Clin. Endocrinol. 90, 145 (2019)

    Article  Google Scholar 

  2. C. Ohlsson, E. Barrett-Connor, S. Bhasin, E. Orwoll, F. Labrie, M.K. Karlsson, Ö. Ljunggren, L. Vandenput, D. Mellström, Å. Tivesten, J. Am. Coll. Cardiol. 58, 1674 (2011)

    Article  CAS  Google Scholar 

  3. T.S. Tran, J.R. Center, M.J. Seibel, J.A. Eisman, M.M. Kushnir, A.L. Rockwood, T.V. Nguyen, Clin. Chem. 61, 1182 (2015)

    Article  CAS  Google Scholar 

  4. Z. Chen, X. Shen, K. Tian, Y. Liu, S. Xiong, Q. Yu, L. Dai, Y. Shi, R. Zhang, R. Zeng, Q. Wan, C. Xiong, Y. Zhou, J. Int. Med. Res. 48, 300060520941715 (2020). https://doi.org/10.1177/0300060520941715

    Article  CAS  PubMed  Google Scholar 

  5. L. Liu, M. Wang, X. Yang, M. Bi, L. Na, Y. Niu, Y. Li, C. Sun, Clin. Chem. 59, 1338 (2013)

    Article  CAS  Google Scholar 

  6. Y. Moriyama, H. Yasue, M. Yoshimura, Y. Mizuno, K. Nishiyama, R. Tsunoda, H. Kawano, K. Kugiyama, H. Ogawa, Y. Saito, K. Nakao, J. Clin. Endocrinol. Metab. 2000, 85 (1834)

    Google Scholar 

  7. D. Lee, H. Kim, S.H. Ahn, S.H. Lee, S.J. Bae, E.H. Kim, H.K. Kim, J.W. Choe, B.J. Kim, J.M. Koh, Clin. Endocrinol. 83, 173 (2015)

    Article  CAS  Google Scholar 

  8. T. Michikawa, Y. Nishiwaki, M. Nakano, S. Iwasawa, M. Yamada, K. Asakura, N. Yoshioka, E. Kuwahara, T. Takebayashi, Am. J. Geriatr. Psychiatry. 21, 1154 (2013)

    Article  Google Scholar 

  9. Ž Debeljak, I. Marković, J. Pavela, I. Lukić, D. Mandić, S. Mandić, V. Horvat, V. Šerić, Biochem. Med. 30, 030701 (2020). https://doi.org/10.11613/BM.2020.030701

    Article  Google Scholar 

  10. K.-I. Ohno, T. Hasegawa, T. Tamura, H. Utsumi, K. Yamashita, Anal. Sci. 34, 1017 (2018)

    Article  CAS  Google Scholar 

  11. H. Kishi, T. Kumazaki, S. Kitagawa, H. Ohtani, Analyst 144, 2922 (2019)

    Article  CAS  Google Scholar 

  12. T. Higashi, S. Ogawa, J. Chromatogr. A 1634, 461679 (2020). https://doi.org/10.1016/j.chroma.2020.461679

    Article  CAS  PubMed  Google Scholar 

  13. S. Ogawa, H. Kittaka, A. Nakata, K. Komatsu, T. Sugiura, M. Satoh, F. Nomura, T. Higashi, J. Pharm. Biomed. Anal. 136, 126 (2017)

    Article  CAS  Google Scholar 

  14. V.K. Narayana, V.M. Tomatis, T. Wang, D. Kvaskoff, F.A. Meunier, Chem. Biol. 22, 1552 (2015)

    Article  CAS  Google Scholar 

  15. S.E. Chen, S. Zhu, J. Hu, J. Sun, Z. Zheng, X.E. Zhao, H. Liu, Anal. Chim. Acta 1124, 40 (2020)

    Article  CAS  Google Scholar 

  16. J. Zheng, S.J. Zheng, C.B. Qi, D.M. Wu, Y.Q. Feng, Anal. Chem. 91, 11440 (2019)

    Article  CAS  Google Scholar 

  17. M. Kamemura, M. Yokota, S. Ogawa, T. Sugiura, T. Higashi, J. Chromatogr. B 1146, 122117 (2020). https://doi.org/10.1016/j.jchromb.2020.122117

    Article  CAS  Google Scholar 

  18. A. Jo, S. Koyagi, W. Hobo, S. Otani, S. Ogawa, T. Higashi, Anal. Sci. 36, 1099 (2020)

    Article  CAS  Google Scholar 

  19. S. Aso, S. Ogawa, S. Nishimoto-Kusunose, M. Satoh, T. Ishige, F. Nomura, T. Higashi, Biomed. Chromatogr. 35, e5027 (2021). https://doi.org/10.1002/bmc.5027

    Article  CAS  PubMed  Google Scholar 

  20. J.D. Colletti, M.M. Redor-Goldman, A.E. Pomperada, A.K. Ghoshal, W.W. Wu, M.J. McPhaul, N.J. Clarke, Clin. Chem. 66, 1181 (2020)

    Article  Google Scholar 

  21. T. Higashi, M. Akaishi, M. Yokota, T. Suzuki, S. Ogawa, Y. Sugiura, T. Nishikawa, K. Nishimoto, M. Suematsu, J. Chromatogr. B 1092, 106 (2018)

    Article  CAS  Google Scholar 

  22. R. Thakare, Y.S. Chhonker, N. Gautam, J.A. Alamoudi, Y. Alnouti, J. Pharm. Biomed. Anal. 128, 426 (2016)

    Article  CAS  Google Scholar 

  23. A.J. Frey, Q. Wang, C. Busch, D. Feldman, L. Bottalico, C.A. Mesaros, I.A. Blair, A. Vachani, N.W. Snyder, Steroids 116, 60 (2016)

    Article  CAS  Google Scholar 

  24. P.J. Crick, J. Aponte, T.W. Bentley, I. Matthews, Y. Wang, W.J. Griffiths, Biochem. Biophys. Res. Commun. 446, 756 (2014)

    Article  CAS  Google Scholar 

  25. G. Sun, J. Xue, L. Li, X. Li, Y. Cui, B. Qiao, D. Wei, H. Li, Mol. Med. Rep. 22, 1576 (2020)

    Article  CAS  Google Scholar 

  26. M.M. Kushnir, A.L. Rockwood, W.L. Roberts, E.G. Pattison, A.M. Bunker, R.L. Fitzgerald, A.W. Meikle, Clin. Chem. 52, 120 (2006)

    Article  CAS  Google Scholar 

  27. G. Eisenhofer, M. Peitzsch, D. Kaden, K. Langton, C. Pamporaki, J. Masjkur, G. Tsatsaronis, A. Mangelis, T.A. Williams, M. Reincke, J.W.M. Lenders, S.R. Bornstein, Clin. Chim. Acta 470, 115 (2017)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 20K06990.

Author information

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan

    Shunsuke Fujimura, Takenori Ito, Shoujiro Ogawa, Shoichi Nishimoto-Kusunose & Tatsuya Higashi

  2. Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Sanzo, Gakuen-cho, Fukuyama, 729-0292, Japan

    Shoujiro Ogawa

  3. Division of Laboratory Medicine, Chiba University Hospital, 1‒8‒1 Inohana, Chuo, Chiba, 260-8677, Japan

    Takayuki Ishige

Authors
  1. Shunsuke Fujimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takenori Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shoujiro Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takayuki Ishige
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shoichi Nishimoto-Kusunose
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tatsuya Higashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tatsuya Higashi.

Additional information

Advanced Publication Released Online by J-STAGE October 1, 2021.

Supplementary Information

Below is the link to the electronic supplementary material.

44211_2022_37_MOESM1_ESM.docx

Supplementary file1 The ESI–MS/MS parameters, method validation procedures and data, and non-derivatization method are available free of charge on the Web at http://www.jasc.or.jp/analsci/. (DOCX 82 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fujimura, S., Ito, T., Ogawa, S. et al. An LC/MS/MS method for quantifying testosterone and dehydroepiandrosterone sulfate in four different serum samples during a single run. ANAL. SCI. 38, 167–173 (2022). https://doi.org/10.2116/analsci.21P268

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.21P268

Keywords

Search

Navigation