Abstract
Shoot tip, bud, leaf, stem and root explants from bearing trees, offshoots, seedlings, and asexual plantlets ofPhoenix dactylifera L. were cultured on modified Murashige and Skoog nutrient media containing 3 g/l activated charcoal, 100 mg/l 2,4-dichlorophenoxyacetic acid, 3 mg/l N 6-(Δ2-isopentyl)adenine to obtain callus. Differential morphogenetic responses were obtained from calli dependent on the explant type and parent source. Subcultured shoot tips and leafy lateral buds callus on nutrient media devoid of charcoal and supplemented with 0.1 mg/l α-naphthaleneacetic acid (NAA) produced adventitious plantlets. Subcultured leaf calli produced roots only. Root callus failed to exhibit any morphogenetic response upon subculturing. Undifferentiated non-leafy buds and stem tissues did not give rise to callus, regardless of the parent source. Generally, the best callus and embryogenetic responses from explants were obtained from seedling and plantlet parent sources. Similarly, organogenetic responses such as root formation and shoot development from shoot tips cultured on media containing 10 mg/l NAA were also related to the parent explant source.
Similar content being viewed by others
References
Apavatjrut, P. andJ. Blake. 1977. Tissue culture of stem explants of coconut (Cocos nucifera L.). Oléagineux32: 267–271.
De Fossard, R.A. 1976 Tissue Culture for Plant Propagators. Univ. of New England, Armidale.
De Guzman, E.V., A.G. del Rosario andE.M. Ubalde. 1979. Proliferative growths and organogenesis in coconut embryo and tissue cultures. Philippine J. Coconut Studies7: 1–10.
Eeuwens, C.J. 1978. Effects of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera L.) and date palms (Phoenix dactylifera L.) cultured in vitro. Physiol. Plant.42: 173–178.
Fisher, J.B. andJ.H. Tsai. 1978. In vitro growth of embryos and callus of coconut palm. In Vitro4: 307–311.
Fulford, R.M., S.H. Justin and A.J. Pussey. 1976. Vegetative propagation of cocunuts. Rep. E. Malling Res. Stn. for 1975. Abstract #06009.
Hasegawa, P.M., T. Murashige andF.H. Takatori. 1973. Propagation of asparagus through shoot apex culture. II. Light and temperature requirements, transplantability of plants, and cyto-histological characteristics. J. Amer. Soc. Hort. Sci.98: 143–148.
Jones, L.H. 1974. Propagation of clonal oil palm tissue culture. Oil Palm News17: 1–9.
Kumar, P. 1980. Role of sex in the production and differentiation of callus on stem cuttings ofTrewia nudiflora Linn. Indian J. Exp. Biol.18: 432–433.
Luxova, M. 1971. Concerning variability in the delimination of the vascular system in root primordia of maize embryos. Biologia Plant.13: 79–87.
Murashige, T. andF. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 473–497.
Rabechault, H. andJ.P. Martin. 1976. Multiplication végétative du Palmier à huile (Elaeis guineensis Jacq.) á l'aide de cultures de tissue foliares. C.R. Acad. Sci. Paris. Série D.283: 1735–1737.
Reuveni, O., Y. Adato andJ. Lillen-Kipnis. 1972. A study of new and rapid methods for the vegetative propagation of data palms. Date Growers's Inst. Rep.49: 17–24.
—. 1974. Studies of thein vitro culture of date palm (Phoenix dactylifera L.) tissues and organs. The Volcani Inst. Agric. Pamphlet. No. 145, p. 1–40.
Schroeder, C.A. 1970. Tissue culture of date shoots and seedlings. Date Grower's Inst. Rep.47: 25–27.
Sharma, D.R., R. Kumari andJ.B. Chowdhury. 1980. In vitro culture, of female date palm (Phoenix dactylifera L.) tissues. Euphytica29: 169–174.
Smith, W.K. andJ.A. Thomas. 1973. The isolation andin vitro cultivation of cells ofElaeis guineensis. Oléagineux28: 123–127.
Staritsky, G. 1970. Tissue culture of the oil palm (Elaeis guineensis Jacq.) as a tool for its vegetative propagation. Euphytica19: 288–292.
Tisserat, B. 1979. Propagation of date palm (Phoenix dactylifera L) in vitro. J. Exp. Bot.30: 1275–1283.
Yang, H.J. andW.J. Clore. 1973. Rapid vegetative propagation of asparagus through lateral bud culture. Hort. Sci.3: 141–143.
Author information
Authors and Affiliations
Additional information
Mention of a trademark or proprietary product in the paper does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be available.
Rights and permissions
About this article
Cite this article
Zaid, A., Tisserat, B. Morphogenetic responses obtained from a variety of somatic explant tissues of data palm. Bot Mag Tokyo 96, 67–73 (1983). https://doi.org/10.1007/BF02491091
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02491091