Abstract
Somatic chromosomes (2n=24) ofPinus luchuensis Mayr at metaphase were observed by fluorescent banding methods with chromomycin A3 (CMA) and DAPI. CMA-bands appeared at the interstitial and/or proximal regions of nearly all chromosomes. DAPI-bands appeared at the interstitial and/or centromeric regions of nearly all chromosomes, and pairs of DAPI-dots appeared at the centromeric regions. Each homologous pair of chromosomes in the chromosome complement was identified by the CMA and DAPI fluorescent banding patterns. The interstitial CMA-bands were mostly localized at the secondary constrictions of the Feulgen-stained chromosomes. The fluorescent banding pattern ofP. luchuensis was very similar to that ofP. thunbergii, but was different from that ofP. densiflora.
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Abbreviations
- CMA:
-
chromomycin A3
- DAPI:
-
4′, 6-diamidino-2-phenylindole
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Hizume, M., Arai, M. & Tanaka, A. Chromosome banding in the genusPinus . Bot Mag Tokyo 103, 103–111 (1990). https://doi.org/10.1007/BF02489619
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DOI: https://doi.org/10.1007/BF02489619