Abstract
Effects of various auxins on callus induction (dedifferentiation) and organ redifferentiation from the callus were studied by using various tissues of rice,Oryza sativa L. cv. Kyoto Asahi.
2,4-D, NAA and IAA were used as auxins for the test of their ability to induce callus. All of these were active. This callus induction by auxin was successful in all tissues used; seed, root, shoot nodule, anther and ovary.
In all of the calluses induced by various auxins such as 2,4-D, NAA and IAA and derived from various tissues such as seed, root, shoot nodule, anther and ovary, organ redifferentiation, i.e., formation of shoots and roots was achieved by removing the auxins from the medium used for the callus calture. Cytokinins were not necessary for the organ redifferentiation in these calluses.
These results suggest that auxin is the only exogenous factor that determines dedifferentiation and redifferentiation in rice plant tissues culturedin vitro.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- IAA:
-
indole-3-acetic acid
References
Furuhashi, K. andM. Yatazawa. 1964. Indentifinitely growing rice callus in tissue culture. Kagaku34: 623 (in Japanese).
Galston, A.W. andL.Y. Dalberg. 1954. The adaptive formation and physiological significance of indole acetic acid oxidase. Amer. J. Bot.41: 373–380.
Kawata, S. andA. Ishihara. 1968. The regeneration of rice plants,Oryza sativa L., in the callus from the seminal root. Proc. Japan Acad.44: 549–552.
Linsmaier, E.M. andF. Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant.18: 100–127.
Maeda, E. 1965. Callus formation and isolation of single cells from rice seedlings. Proc. Crop Sci. Soc. Japan34: 139–147.
— 1968. Subculture and organ formation in the callus derived from rice embryosin vitro. Proc. Crop. Sci. Soc. Japan37: 51–58.
Niizeki, H. andK. Oono. 1968. Induction of haploid rice plants from anther cultures. Proc. Japan Acad.44: 554–557.
Nishi, T., Y. Yamada andE. Takahashi. 1968. Organ redifferentiation and plant restoration in rice callus. Nature219: 508–509.
— andS. Mitsuoka. 1969. Occurrence of various ploidy plants from anther and ovary cultures of rice plants. Jap. J. Genetics44: 341–346.
Saka, H. andE. Maeda. 1969. Effect of kinetin on organ formation in callus tissue derived from rice embryos. Proc. Crop Sci. Soc. Japan38: 668–674.
Skoog, F. andC.O. Miller. 1957. Chemical regulation of growth and organ formation in plant tissues culturedin vitro. Symp. Soc. Exptl. Biol.11: 118–131.
Tamura, S. 1968. Shoot formation in callus orginated from rice embryo. Proc. Japan Acad.44: 544–548.
Thimann, K.V. 1934. Studies on the growth hormone of plants VI. The distribution of the growth substance in plant tissues. J. Gen. Physiol.18: 23–34.
van Overbeek, J., E. S. de Vázquez andS.A. Gordon. 1947. Free and bound auxin in the vegetative pineapple plant. Amer. J. Bot.34: 266–270.
Yamada, Y., K. Tanaka andE. Takahashi. 1967. Callus induction in rice,Oryza sativa L. Proc. Japan Acad.43: 156–160.
—,T. Nishi, T. Yasuda andE. Takahashi. 1968. The sterile culture of rice cells,Oryza sativa L. and its application.In: M. Miyakawa and T.D. Luckey, ed., Advances in Germfree Research and Gnotobiology pp. 377–386, Illiffe Books Ltd., London.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nishi, T., Yamada, Y. & Takahashi, E. The role of auxins in differentiation of rice tissues culturein Vitro . Bot Mag Tokyo 86, 183–188 (1973). https://doi.org/10.1007/BF02489225
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02489225