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The metabolism ofStreptomyces griseus

XI. Further observation on the electron transfer system of theStreptomyces griseus respiratory particle

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Abstract

The electron transfer pathway in the respiratory particles ofStreptomyces griseus was studied. Vitamins K3 and K5,α- andβ-naphthoquinones, served as the hydrogen acceptors in succinate oxidation, and succinate- and reduced nicotinamide adenine dinucleotide (NADH)-cytochromec reductase activities, but were ineffective for NADH oxidase activity. Vitamin K seemed to mediate the hydrogen from NADH-diaphorase to cytochromec. Chlorpromazine inhibited electron transfer in the respiratory particles. Cyanide completely inhibited the electron transfer system initially, however, oxygen consumption increased gradually with time. AlthoughS. griseus possesses cytochromesa, b, c and pigment 625 (probablyd), the electron transfer chain was complicated. Two terminal oxidase activities (cytochromec oxidase and cytochromec peroxidase activities) were detected in the respiratory particles ofS. griseus.

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Authors and Affiliations

  1. Kaken Chemical Company Ltd., Honkomagome, Bunkyo-ku, 113, Tokyo

    Yukio Inoue

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  1. Yukio Inoue
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Dedicated to Prof. Shoichiro Usami celebrating his sexagenary birthday.

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Inoue, Y. The metabolism ofStreptomyces griseus . Bot Mag Tokyo 86, 121–132 (1973). https://doi.org/10.1007/BF02488520

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  • DOI: https://doi.org/10.1007/BF02488520

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