Abstract
Spores ofAdiantum capillus-veneris L., which were preincubated at 25 C for three days in the dark, were suspended in 1 mM potassium phosphate buffer, pH 6.0, and incubated for four days under continuous red light in the presence or absence of 3 mM sulfite. At day 0, 2 and 4 of the incubation, contents of cell constituents were determined. Total lipid content decreased continuously over four days of incubation in the absence of sulfite or in the presence of 3 mM sulfate. In contrast, when sulfite was added to the medium, the decrease stopped after day 2. The content of insoluble glucan increased markedly between day 2 and 4 in the medium without sulfite, whereas it decreased continuously for four days in the medium containing sulfite. The protein content decreased promptly by day 2, but its decrease was delayed when 3 mM sulfite was added to the medium. The content of amino acids also decreased by day 2, but it increased thereafter in the absence of sulfite or in the presence of 3 mM sulfate. In the presence of sulfite, however, the content continued to decrease until day 4. The results indicate that 3 mM sulfite in the incubation medium depressed the utilization of reserve lipid and protein, the synthesis of insoluble glucan and the increase of amino acid pool sizes in fern spores.
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Abbreviations
- KPB:
-
Potassium phosphate buffer (pH 6.0)
- SDS-PAGE:
-
Sodium dodecylsulfate-polyacrylamide gel electrophoresis
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Minamikawa, T., Masuda, N., Kadota, A. et al. Effect of sulfite on compositional changes of cell constituents in germinating spores ofAdiantum capillus-veneris L.. Bot. Mag. Tokyo 100, 1–8 (1987). https://doi.org/10.1007/BF02488415
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DOI: https://doi.org/10.1007/BF02488415