Abstract
Phosphodiester cleavage of BNPP can be accelerated by various degradative receptors including enzymes. In the hydrolytic cleavage of BNPP, a bell-shaped receptor concentration-rate profile composed of a positive-catalysis phase at low receptor concentrations and a negative-catalysis phase at excess receptor concentration was observed. The negative catalysis can be explained by nonproductive binding, leading to an increase of the activation energy barrier and a decrease of the reaction rate.
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Ryu, HW., Cho, Y., Kim, DY. et al. Accelerated phosphodiester cleavage of bis(p-nitrophenyl)-phosphate by receptors. React Kinet Catal Lett 66, 127–132 (1999). https://doi.org/10.1007/BF02475751
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DOI: https://doi.org/10.1007/BF02475751