Abstract
The activity of tyrosine hydroxylase, the key enzyme in catecholamine biosynthesis, was studied along with adrenoceptor density in the brains of male CBA/Lac, BALB/cLac, and C57BL/6J mice, which show different responses to novel environments. C57BL mice showed the highest level of movement activity and the lowest level of emotionality in a novel environment. Mice of this line also showed the highest brainstem tyrosine hydroxylase activity. At the same time, the density of β-adrenoceptors in the cortex and hypothalamus of C57BL mice was ower than in the other two lines of mice, while the density of α2-adrenoceptors in these parts of the brain was lower than in CBA mice. In BALB mice, movement activity was twice as high as in CBA mice, while levels of emotionality were similar in these two lines. Tyrosine hydroxylase activity was higher in the cerebral cortex of BALB mice, while the density of α2-adrenoceptors was lower than in CBA mice. These results show that increased investigative activity and decreased emotionality were seen in animals with higher levels of noradrenaline synthesis and decreased density of adrenergic receptors in the brain.
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References
N. N. Dygalo, “A simple, sensitive modification of a fluorimetric method for assaying the activity of tyrosine hydroxylase in brain tissues,”Vopr. Med. Khimii,33, No. 4, 126–129 (1987).
G. T. Shishkina and N. N. Dygalo, “The molecular physiology of adrenergic receptors,”Usp. Fiziol. Nauk.,28, No. 1, 61–74 (1997).
N. E. Anden, K. Pauksens, and K. Svensson, “Selective blockade of brain alpha2-autoreceptors by yohimbine: effects of motor activity and on turnover of noradrenaline and dopamine,”J. Neural Transm.,55, 111–120 (1982).
H. Anisman and R. H. Zacharko, “Behavioral and neurochemical consequences associated with stressors,”Ann. N.Y. Acad. Sci.,467, 205–225 (1986).
L. Cervo and R. Samanin, “Clonidine causes antidepressant-like effects in rats by action on alpha2-adrenoceptors outside the locus coeruleus,”Eur. J. Pharmacol.,193, 309–313 (1991).
N. Ginovart, D. Marcel, L. Bezin, C. Gagne, J. F. Pujol, and D. Weissmann, “Tyrosine hydroxylase expression within Balb/C and C57black/6 mouse locus coeruleus. II. Quantitative study of the enzyme level,”Brain Res.,719, 45–55 (1996).
A. L. Gorman and A. J. Dunn, “Beta-adrenergic receptors are involved in stress-related behavioral changes,”Pharmacol. Biochem. Behav.,45, 1–7 (1993).
J. Haller, G. B. Makra, I. Pinter, I. Gyertyan, and A. Egyed, “The mechanism of action of alpha2-adrenoceptor blockers as revealed by effects on open field locomotion and escape reactions in the shuttle-box,”Psychopharmacology (Berlin),134, 107–114 (1997).
S. Nomura, J. Shimizu, N. Ukei, S. Sakaida, and T. Nakazawa, “The activation of mice's behavior and reduction of brain beta-adrenergic receptor binding following repeated administration of antidepressant drugs,”Yakubutsu Seishin Kodo,4, 237–241 (1984).
S. Nomura and K. Yamaoka, “Antidepressant drugs and beta-adrenergic receptors,”Yakubutsu Seishin Kodo,8, 437–441 (1988).
M. Palkovits and M. J. Brownstein, “Catecholamine in the central nervous system,” in:Handbook of Experimental Pharmacology, Springer, Berlin (1989), pp. 1–26.
S. J. Sara, C. Dyon-Laurent, and A. Herve, “Novelty-seeking behavior in the rat is dependent upon the integrity of the noradrenergic system,”Brain Res. Cogn. Brain,2, 181–187 (1995).
G. T. Shishkina and E. V. Naumenko, “Correlation between the hypothalamic density of3H-clonidine-binding sites and plasma testosterone levels in mice,”Neuroendocrinology,61, 663–668 (1995).
E. A. Stone, J. Rhee and D. Quartermain, “Blockade of effect of stress on risk assessment behavior in mice by a beta-1 adrenoceptor antagonist,”Pharmacol. Biochem. Behav.,55, 215–217 (1996).
R. E. Zigmond, M. A. Schwarzschild, and A. R. Rittenhouse, “Acute regulation of tyrosine hydroxylase activity by nerve activity and by neurotransmitters via phosphorylation,”Ann. Rev. Neurosci.,12, 415–461 (1989).
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Translated from Rosiikii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 1, pp. 105–109, January, 1999.
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Dygalo, N.N., Shishkina, G.T. Genetic differences in the synthesis and reception of noradrenaline in the mouse brain and behavior in a novel environment. Neurosci Behav Physiol 30, 327–330 (2000). https://doi.org/10.1007/BF02471786
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DOI: https://doi.org/10.1007/BF02471786