Skip to main content
SpringerLink
Log in

Effects of the oral or intratumoral administration of OK432 on the immuno-reactivities of regional lymph nodes in gastric cancer patients

  • Original Articles
  • Published:
The Japanese journal of surgery Aims and scope Submit manuscript

Abstract

The effects of OK432, a streptococcal preparation, administered either orally (PO-OK432) or intratumorally (IT-OK432) on the immuno-reactivities of regional lymph nodes were investigated in gastric cancer patients. Although native lymph node lymphocytes (LNL) from untreated patients did not show any cytotoxicities against K562 and Raji cells, enhanced activities were found in LNL from patients administered OK432. Augmenting effects on the cytotoxicities of LNL byin vitro additional OK432, interleukin 2 or γ-interferon were remarkable in the patients given IT-OK432. Moreover, the cytotoxicities of peripheral blood lymphocytes were augmentedin vitro more strongly in patients given IT-OK432 than in those given PO-OK432. Flow cytometric analysis of LNL revealed a decrease in CD4+ cells by PO-OK432 and an increase in CD8+ cells by IT-OK432. An increase in CD4+2H4+ cells and a decrease in CD4+2H4− cells were observed in the patients given OK432, though CD8+CD11+ cells decreased by PO-OK432 while CD8+CD11+ cells increased by IT-OK432. Thus, it is suggested that LNL reactive to OK432 immunotherapy may differ between PO- and IT-OK432, and that the immunoreactivities of local lymph nodes and systemical immuno-reactivities may be highly potentiated by IT-OK432 rather than PO-OK432.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Colotta F, Rambaldi A, Colombo N, Tabacchi L, Introna M, Mantovani A. Effect of a streptococcal preparation (OK-432) on natural killer activity of tumor-associated lymphoid cells in human ovarian carcinoma and on lysis of fresh ovarian tumor cells. Br J Cancer 1983; 48: 515–525.

    PubMed  CAS  Google Scholar 

  2. Katano M, Torisu M. Neutrophil-mediated tumor cell destruction in cancer ascites. Cancer 1982; 50: 62–68.

    Article  PubMed  CAS  Google Scholar 

  3. Yanagawa E, Toge T, Niimoto M, Hattori T.In vitro augmentation of natural and autotumor killing activity of tumor-associated macrophages by OK-432, interleukin-2 and interferon. In: Hoshino T, ed. Immunopharmacological aspects of OK-432 in humans. Tokyo: Excerpta Medica 1986; 17–26.

    Google Scholar 

  4. Nakazawa S, Yoshino J, Ohta H. Endoscopic injection of OK-432 into gastric cancer in combination with systemic immunochemotherapy: A clinical trial. In: Tobe T, ed. New applications of OK-432. Tokyo: Excerpta Medica 1986; 55–65.

    Google Scholar 

  5. Niimoto M, Kameda A, Yanagawa E, Toge T, Hattori T. Intratumoral large-dose administration of OK-432 in patients with advanced gastric cancer. In: Ota K, ed. Clinical application of OK-432 for control of cancer. Tokyo: Excerpta Medica 1986; 18–25.

    Google Scholar 

  6. Toge T, Kuninobu H, Yamaguchi Y, Baba N, Kegoya Y, Hattori T. The clinical efficacy of intratumoral OK-432 administration in advanced cancer patients. Jpn J Surg 1988; 18: 668–674.

    Article  PubMed  CAS  Google Scholar 

  7. Tan M, Akimoto M, Abe R, Kasai M, Ishii H, Sano S, Fujimoto H. Clinical trials of oral administration of OK-432 in cancer patients. In: Tobe T, ed. New applications of OK-432. Tokyo: Excerpta Medica 1986; 47–54.

    Google Scholar 

  8. Müller-Schoop JW, Good RA. Functional studies of Peyer's patches: Evidence for their participation in intestinal immune response. J Immunol 1975; 114: 1757–1760.

    PubMed  Google Scholar 

  9. Nio Y, Tsuchitani T, Ohgaki K, Inamoto T, Kan N, Tobe T. Effects of oral administration of the streptococcal preparation OK-432 (Picibanil) on natural killer cell activity and lymphoproliferative response in mice with transplanted cecal tumors. In: Tobe T, ed. New applications of OK-432. Tokyo: Excerpta Medica 1986; 1–15.

    Google Scholar 

  10. Moore M, Vose BM. Extravascular natural cytotoxicity in man: Anti-K562 activity of lymph-node and tumor-infiltrating lymphocytes. Int J Cancer 1981; 27: 265–272.

    PubMed  CAS  Google Scholar 

  11. Yanagawa E, Uchida A, Micksche M. Natural cytotoxicity of lymphocytes from lymph nodes draining breast carcinoma and its augmentation by interferon and OK432. Cancer Immunol Immunother 1984; 17: 1–6.

    Article  PubMed  CAS  Google Scholar 

  12. Japanese Society for Gastric Cancer. The general rules for gastric cancer study in surgery and pathology. Jpn J Surg 1981; 11: 127–139.

    Article  Google Scholar 

  13. Cunningham-Rundles S, Filippa DA, Braun DW Jr, Antonelli P, Ashikari H. Natural cytotoxicity of peripheral blood lymphocytes and regional lymph node cells in breast cancer in women. J Natl Cancer Inst 1981; 67: 585–590.

    PubMed  CAS  Google Scholar 

  14. Cunningham-Rundles S. Control of natural cytotoxicity in the regional lymph node in breast cancer. In: Herberman RB, ed. NK cells and other effector cells. New York: Academic Press 1982; 1133–1140.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Department of Surgery, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi 1-2-3, Minami-ku, 734, Hiroshima, Japan

    Etsuro Yanagawa MD, Tetsuya Toge, Yoshiyuki Yamaguchi, Hiroshi Kuninobu, Katsumasa Kuroi, Yasuhide Kegoya, Nobutoshi Baba, Takahiro Takayama & Yukio Sato

Authors
  1. Etsuro Yanagawa MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuya Toge
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshiyuki Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroshi Kuninobu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Katsumasa Kuroi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yasuhide Kegoya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nobutoshi Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Takahiro Takayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yukio Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yanagawa, E., Toge, T., Yamaguchi, Y. et al. Effects of the oral or intratumoral administration of OK432 on the immuno-reactivities of regional lymph nodes in gastric cancer patients. The Japanese Journal of Surgery 21, 297–303 (1991). https://doi.org/10.1007/BF02470950

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02470950

Key Words

Search

Navigation