Summary
Conditions were determined for the separation of a complex set of anthocyanins (free aglycones, mono- and multiglycosides and esterified forms) by HPLC. The optimised gradient elution method was then used to carry out qualitative and quantitative analysis of anthocyanin compounds present in the callus tissue ofRudbeckia hirta L. and the tubular flowers of the soil-based plant. The summary content of anthocyanin pigments and the content of the main pigment was identified in the analysed biomass. The method developed is useful for the purposes of monitoring the process of biosynthesis of anthocyanins in tissues obtained through in vitro cultures. The advantages of the method for anthocyanins and its application to other anthocyanin-rich materials are also discussed.
Similar content being viewed by others
References
D. Pathak, K. Pathak, A. K. Singla, Fitoterapia62, 371 (1991).
J. K. Podlewska, A. Chwalibowska-Podlewska, Leki współczesnej terapii, Büchner Fundation, Warszawa, 1992, 534.
N. Saito, T. S. Lu, M. Yokoi, A. Shigihara, T. Honda, Phytochemistry33, 245 (1993).
N. Saito, J. B. Harborne, Phytochemistry31, 3009 (1992).
J. Šicha, H. Becker, Pharmazie46, 363 (1991).
A. Baj, E. Bombardelli, B. Gabetta, E. M. Martinelli, J. Chromatogr.279, 366 (1983).
N. Saito, J. Cheng, M. Ichimura, M. Yoko, Y. Abe, T. Honda, Phytochemistry35, 687 (1994).
K. Yoshida, T. Kondo, T. Goto, Tetrahedron Letters48, 4313 (1992).
O. Dangles, N. Saito, R. Broullard, J. Am. Chem. Soc.115, 3125 (1993).
G. Matysik, J. Plan. Chromatogr.5, 146 (1992).
G. Matysik, M. Benesz, Chromatographia32, 19 (1991).
A. Cheminat, R. Brouillard, P. Guerne, P. Bergman, B. Refher, Phytochemistry28, 3246 (1991).
H. S. Lee, V. Hong, J. Chromatogr.624, 221 (1992).
K. Hosokawa, Y. Fukunaga, E. Fukushi, J. Kawabata, Phytochemistry41, 1531 (1991).
O. M. Andersen, G. Francis, J. Chromatogr.318, 450 (1985).
M. Azard, E. Vilette, S. Brauns, J. Food Sci52, 1255 (1987).
I. G. Kirchner in E. E. Perry Ed., Thin-layer Chromatography Techniques of Chemistry. Volume XIV, Sigma Company, 1990, p. 801–827.
P. Fiqueiredo, M. Elhabiri, K. Toki, N. Saito, O. Dangles, R. Brouillard, Phytochemistry37, 301 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Łuczkiewicz, M., Cisowski, W. The RP-HPLC analysis of anthocyanins. Chromatographia 48, 360–364 (1998). https://doi.org/10.1007/BF02467704
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02467704