Summary
The variation in anthocyanins content of the petals of red poppy (Papaver, Rhoeas) during development of the flower from the closed bud stage have been investigated. Six-stage gradient thin-layer chromatography of the polyzonalstepwise type was used, starting from ethyl acetate, the last eluent fraction being isopropanol-water-acetic acid (65∶32∶3). Silica Si 60 was used as adsorbent. The densitograms were recorded at 465 nm; they show gradually increasing peaks of red anthocyanins in the petals of closed buds, partly opened buds and fully developed flower and changes in the proportions of several anthocyanins, with various RF values, during the growth of the flower.
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Editor's note: see also J. P. Goiffon, M. Brun and M.-J. Bourrier, J. Chromatogr.517, 101 (1991)
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Matysik, G., Benesz, M. Thin-layer chromatography and densitometry of anthocyanins in the petals of red poppy during development of the flowers. Chromatographia 32, 19–22 (1991). https://doi.org/10.1007/BF02262460
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DOI: https://doi.org/10.1007/BF02262460