Summary
Solid phase extraction (SPE) methods are studied for the sample pretreatment for HPLC analysis ofTaxus cell suspension cultures. Various types of SPE materials were tested for the extraction of both the taxane standards and samples of various origin. Comparison between the different cartridges and the different elution solvents are made in terms of extraction recovery and sample clean-up. Selective elution of the taxanes is achieved by a gradient elution scheme.
Similar content being viewed by others
References
G. M. Cragg, S. A. Schepartz M. Suffness, M. R. Grever, J. Nat. Prod.56, 1657 (1993).
A. G. Fett-Netto, F. DiCosmo, W. F. Reynolds, K. Sakata, Biotechnology10, 1572 (1992).
J. H. Kim, J. H. Yun, Y.-S. Hwang, S. Y. Byun, D. II Kim, Biotechnol. Lett.17, 101 (1995).
E. R. M. Wicremeninhe, R. N. Arteca, Plant Cell Tiss. Org. Cult.,35, 181 (1993).
D. M. Gibson, R. E. B. Ketchum, N. C. Vance, A. A. Christen: Plant Cell Rep.12, 479 (1993).
T. J. Hirasuna, L. J. Pestchanker, V. Shrinivasan, M. L. Shuler, Plant Cell Tiss. Org. Cult.44, 95–102 (1996).
N. Mirjalili, J. C. Linden, Biotechnol. Bioeng.48, 123 (1995).
V. Ciddi, V. Srinivasan, M. L. Shuler, Biotechnol. Lett17, 1343 (1995).
V. Shrinivasan, V. Ciddi, V. Bringi, M. L. Shuler, Biotechnol. Prog.12, 457 (1996).
Y. Yukimune, H. Tabata, Y. Higashi, Y. Hara, Nat. Biotechnol.,14, 1129 (1996).
P. Heinstein, J. Yun Zhou, M. Wang, Y. C. Liu, X. Ya. Chen, D. Chen, S. Hoke, R. G. Cooks, C. Chang, J. Chem. Soc. Perkin Trans.1, 845 (1996).
A. Zhiri, M. Jaziri, Y. Guo, R. Vanhaelen-Fastre, M. Vanhaelen, J. Homes, K. Yoshimatsu, K. Shimomura, Biol. Chem. Hoppe Seyler,376, 583 (1995).
R. E. B. Ketchum, D. M. Gibson, Plant Cell Tiss. Org. Cult.,46, 9 (1996).
E. R. M. Wickremesinhe R. N. Arteca, J. Plant Physiol.144, 183 (1994).
M. Jaziri, A. Zhiri, Y. W. Guo, J.-P. Dupont, K. Shimomura, H. Hamada, M. Vanhaelen, J. Homes, Plant Cell Tiss. Org. Cult.46, 59 (1996).
K. M. Witherup, S. A. Look, M. W. Stasko, T. G. McCloud, H. J. Issaq, G. M. Muschik, J. Liq. Chromatogr.12, 2117 (1989).
S. L. Richheimer, D. M. Tinnermeier, D. W. Timmons, Anal. Chem.64, 2323 (1992).
R. E. B. Ketchum, D. M. Gibson, J. Liq. Chromatogr.16, 2519 (1993).
R. E. B. Ketchum, D. M. Gibson, J. Liq. Chromatogr.18, 1093 (1995).
E. R. M. Wicremesinhe, R. N. Arteca, J. Liq. Chromatogr.,16, 3263 (1993).
F. Bitsch, W. Ma, F. MacDonald, M. Nieder, C. H. L. Shackleton J. Chromatogr.615, 273 (1993).
G. Theodoridis, R. Verpoorte, Phytochem. Anal.7, 169 (1996).
K. Glowniak, G. Zgórka, A. Józefczyk, M. Furmanowa, J. Pharm. Biomed. Anal.14, 1215 (1996).
G. Theodoridis, G. Laskaris, C. de Jong, R. Verpoorte, Proceedings on the HPLC '96 symposium p. 233.
G. Theodoridis, G. Laskaris, C. F. de Jong, A. J. P. Hofte, R. Verpoorte, J. Chromatogr. B. Submitted for publication.
O. C. Gamborg, R. A. Miller, K. Ojima. Exp. Cell Res.50, 151 (1968).
G. Theodoridis, G. Laskaris, E. L. M. van Rozendaal, R. Verpoorte, Manuscript in preparation.
G. Theodoridis, I. Papadoyannis, H. Tsoukali-Papadopoulou, G. Vasilikiotis, J. Liq. Chomatogr.18, 1973 (1995).
I. Papadoyannis, A. Zotou, V. Samanidou, G. Theodoridis, F. Zougrou, J. Liq. Chomatogr.16, 3017 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Theodorídis, G., de Jong, C.F., Laskaris, G. et al. Application of SPE for the HPLC analysis of taxanes fromTaxus cell cultures. Chromatographia 47, 25–34 (1998). https://doi.org/10.1007/BF02466782
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02466782