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Chromatographic classification of commercially available reverse-phase HPLC columns

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Summary

The ever-increasing number of commercially available reversed phases with which the analyst is confronted can cause problems in column selection. These and the non-standard test procedures used by the column manufacturers and packing companies cause further confusion. In order to independently compare and contrast a range of well established C18 stationary phases, we have performed a modified column characterization approach, based on Tanaka's methodology, on thirty commonly used phases in our laboratory. These results have been evaluated and presented in various formats as Principal Component Analysis, Cluster Analysis, Tabular Format and Radar Plots in order to assist in observing similarities and differences between phases.

The results indicate that, while no two phases are exactly the same (with the exception of “me-to” clones), it is possible to characterize phases into different classes based on their chromatographic performance against selected test probes. The paper illustrates the similarities and differences between these phases.

The column characterization described in the paper can form the basis of a rational column selection protocol by either careful matching of the appropriate column characteristics to the analyte's physico-chemical properties or by a systematic evaluation of columns from our various categories.

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Authors and Affiliations

  1. Pharmaceutical and Analytical, Research and Development, Astra Charnwood, Bakewell Road, LE11 5RH, Loughborough, Leicestershire, UK

    E. Cruz, M. R. Euerby & C. M. Johnson

  2. Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, UK

    C. A. Hackett

Authors
  1. E. Cruz
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  2. M. R. Euerby
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  3. C. M. Johnson
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  4. C. A. Hackett
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Cruz, E., Euerby, M.R., Johnson, C.M. et al. Chromatographic classification of commercially available reverse-phase HPLC columns. Chromatographia 44, 151–161 (1997). https://doi.org/10.1007/BF02466449

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  • DOI: https://doi.org/10.1007/BF02466449

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