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Hyphal anastomosis and complementary growth of fused cells inAlternaria alternata

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Mycoscience

Abstract

Hyphal anastomosis and complementary growth of fused cells inAlternaria alternata were investigated. Sixty-four experimental isolates were divided into anastomosis-positive (A+) and anastomosis-negative (A−) groups based on their self-anastomosing ability. Nonself-anastomoses (interisolate) were readily distinguished from self-anastomoses (intraisolate) by using a mixed culture of conidia and hyphal fragments prepared from the respective isolates. Nonself-anastomosis occurred only between the A+ isolates irrespective of their pathogenicity and geographic origin. The breakdown of cell walls and the establishment of cytoplasmic continuity between fused cells were microscopically observed only in the self-anastomoses. The frequency of the nonself-anastomosis was, in general, lower than that of the self-anastomosis. For analysis of complementation between the fused cells, mutants doubly marked with auxotrophy and hygromycin B (Hyg) resistance were prepared from wild-type isolates. The identity of the mutants was confirmed by RAPD analysis using three arbitrary primers. Complementary growth occurred only between an A+ isolate and its mutant(s) on a minimal medium containing Hyg, demonstrating that the self-anastomoses resulted in perfect cell fusions and the nonself-anastomoses were contact or imperfect fusions.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Tottori University, 680, Tottori, Japan

    Si-Liang Huang & Hiroshi Otani

  2. Faculty of Agriculture, Tottori University, 680, Tottori, Japan

    Yasuo Itoh, Keisuke Kohmoto & Motoichiro Kodama

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  1. Si-Liang Huang
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  2. Yasuo Itoh
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  3. Keisuke Kohmoto
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  4. Hiroshi Otani
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  5. Motoichiro Kodama
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Huang, SL., Itoh, Y., Kohmoto, K. et al. Hyphal anastomosis and complementary growth of fused cells inAlternaria alternata . Mycoscience 37, 1–13 (1996). https://doi.org/10.1007/BF02461449

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