Skip to main content
Log in

Morphological classification of ectomycorrhizas ofPinus densiflora

  • Original Papers
  • Published:
Mycoscience

Abstract

Morphological classification of ectomycorrhizas ofPinus densiflora was conducted. Fifty soil samples containing pine ectomycorrhizas, and 40 pine seedlings were collected randomly in two separate reforested stands ofP. densiflora (45 yr old) from May 1992 to October 1994. Fifty-six types of ectomycorrhizas could be classified based upon microscopically observable morphological characteristics. Fifty percent of the types showed cystidia or other specific characteristics such as laticiferous hyphae in the fungal sheaths, verrucose emanating hyphae and a positive hyphal reaction to UV irradiation. Four mycorrhizal types were confirmed to be formed by the fungiRussula delica, R. mariae, R. nigricans, andCenococcum geophilum, respectively. Although the other 52 types were unidentified mycobionts at species level, it was inferred that they were formed by the fungiHebeloma, Lactarius, Russula andTuber. There was a slight difference in the observed mycorrhizal types between the tree ages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature cited

  • Agerer, R. 1987–1994. Color atlas of ectomycorrhizas, Einhorn-Verlag, Schwabisch Gmünd.

    Google Scholar 

  • Agerer, R. 1991. Characterization of ectomycorrhiza. In: Methods in microbiology, vol. 23, (ed. by Norris, J. R., Read, D. J. and Varma, A. K.), pp. 25–73. Academic Press, London.

    Google Scholar 

  • Agerer, R. 1995. Anatomical characteristics of identified ectomycorrhizas: an attempt towards a natural classification. In: Mycorrhiza: structure, function, molecular biology and biotechnology, (ed. by Varma, A. and Hock, B.), pp. 685–735. Springer-Verlag, Berlin.

    Google Scholar 

  • Alexander, I. J. 1981. ThePicea sitchensis+Lactarius rufus mycorrhizal association and its effects on seedling growth and development. Trans. Br. Mycol. Soc.76: 417–423.

    Google Scholar 

  • Bruns, T. 1995. Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant Soil170: 63–73.

    Article  CAS  Google Scholar 

  • Chilvers, G. A. 1968. Some distinctive types of eucalypt mycorrhiza. Aust. J. Bot.16: 49–70.

    Article  Google Scholar 

  • Chu-Chou, M. 1979. Mycorrhizal fungi ofPinus radiata in New Zealand. Soil Biol. Biochem.11: 557–562.

    Article  Google Scholar 

  • Danielson, R. M. 1984. Ectomycorrhizal associations in jack pine stands in northeastern Alberta. Can. J. Bot.62: 932–939.

    Google Scholar 

  • Dominik, T. 1969. Key to ectotrophic mycorrhizae. Folia Forest Polonica, Ser. A15: 309–321.

    Google Scholar 

  • Duckett, J. B. and Read, D. J. 1989. The use of the fluorescent dye, 3,3′-dihexyloxacarbocyanine iodide, for the selective staining of ascomycete fungi associated with liverwort rhizoids and ericoid mycorrhizal roots. New Phytol.118: 259–272.

    Article  Google Scholar 

  • Fassi, B. 1965. Ectotrophic mycorrhizae produced byEndogone lactiflua Berk. onPinus strobus L. Allionia11: 7–15.

    Google Scholar 

  • Fontana, A. and Centrella, E. 1967. Ectomycorrhizae produced by hypogeous fungi. Allionia13: 149–176.

    Google Scholar 

  • Godbout, C., and Fortin, J. A. 1985. Synthesized ectomycorrhizae of aspen: fungal genus level structural characterization. Can. J. Bot.63: 252–262.

    Article  Google Scholar 

  • Harley, J. L. and Smith, S. E. 1983. Mycorrhizal symbiosis, Academic Press, London.

    Google Scholar 

  • Hawksworth, D. L., Sutton, B. C. and Ainthworth, G. C. 1983. Ainthworth & Bisby's Dictionary of the fungi, 7th ed., pp. 444–445, CAB, Surrey.

    Google Scholar 

  • Imazeki, R. and Hongo, T. 1987. Colored illustrations of mushrooms of Japan, vol. 1, Hoikusha, Osaka. (In Japanese).

    Google Scholar 

  • Imazeki, R. and Hongo, T. 1989. Colored illustrations of mushrooms of Japan, vol. II, Hoikusha, Osaka. (In Japanese).

    Google Scholar 

  • Ingleby, K., Mason, P. A., Last, F. T. and Fleming, L. V. 1990. Identification of ectomycorrhizas. HMSO, London.

    Google Scholar 

  • Lamb, R. J. and Richards, B. N. 1970. Some mycorrhizal fungi ofPinus radiata andP. eliottii var.eliottii in Australia. Trans. Br. Mycol. Soc.54: 371–378.

    Google Scholar 

  • Masui, K. 1927. A study of the ectotrophic mycorrhizas of woody plants. Mem. Coll. Sci. Kyoto Univ. Ser. B3: 149–279.

    Google Scholar 

  • Mikola, P. 1973. Application of mycorrhizal symbiosis in forest practice. In: Ectomycorrhizae: Their ecology and physiology, ed. by Marks, G. C. and Kozlowski, T. T.), pp. 383–411. Academic Press, London.

    Google Scholar 

  • Molina, R. and Trappe, J. M. 1982. Pattern of ectomycorrhizal host specificity and potential among Pacific northwest conifers and fungi. Forest Sci.28: 423–458.

    Google Scholar 

  • Molina, R., Massicotte, H. and Trappe, J. M. 1992. Specificity phenomena in mycorrhizal symbiosis: Community-ecological consequences and practical implications. In: Mycorhizal functioning, (ed. by Allen, M. F.), pp. 357–423. Chapman and Hall, New York.

    Google Scholar 

  • Newton, A. C. and Pigott, C. D. 1991. Mineral nutrition and mycorrhizal infection of seedling oak and birch III. Epidemiological aspects of ectomycorrhizal infection, and the relationship to seedling growth. New Phytol.117: 53–60.

    Article  Google Scholar 

  • Newton, A. C. 1992. Toward a functional classification of ectomycorrhizal fungi. Mycorrhiza2: 75–79.

    Article  Google Scholar 

  • Palenzona, M., Chevalier, G. and Fontana, A. 1972. Mycorrhizal synthesis between the mycelia in culture ofTuber brumale, T. melanosporum, T. rufum, and conifer and broad-leaves seedlings. Allionia18: 41–52.

    Google Scholar 

  • Pigott, C. D. 1982. Fine structure of mycorrhiza formed byCenococcum geophilum Fr. onTilia cordata Mill. New Phytol.92: 501–512.

    Article  Google Scholar 

  • Satake, Y., Hara, H., Watari, S. and Tominari, T. 1989. Wild flowers of Japan: Woody plants, vol. 1. Heibonsha, Tokyo. (In Japanese.)

    Google Scholar 

  • Taylor, A. F. S. and Alexander, I. J. 1989. Ectomycorrhizal synthesis with an isolate ofRussula aeruginea. Mycol. Res.92: 103–107.

    Article  Google Scholar 

  • Thomas, G. W., Rogers, D. and Jackson, R. M., 1983. Change in the mycorrhizal status of Sitka spruce following outplanting. Plant Soil71: 319–323.

    Article  Google Scholar 

  • Tonkin, C. M., Malajczuk, N. and McComb, J. A. 1989. Ectomycorrhizal formation by micropropagated clones ofEucalyptus marginata inoculated with isolates ofPitholithus tinctorius. New Phytol.111: 209–214.

    Article  Google Scholar 

  • Trappe, J. M. 1964. Mycorrhizal host and distribution ofCenococcum graniforme. Lloydia27: 100–106.

    Google Scholar 

  • Walker, C. 1985.Endogone lactiflua forming ectomycorrhizas withPinus contorta. Trans. Br. Mycol. Soc.84: 353–355.

    Article  Google Scholar 

  • Yamada, A. and Katsuya, K. 1995. Mycorrhizal association of isolates from sporocarps and ectomycorrhizas withPinus densiflora seedlings. Mycoscience36: 315–323.

    Article  Google Scholar 

  • Zak, B. 1973. Classification of ectomycorrhizae. In: Ectomycorrhizae: Their ecology and physiology. (ed. by Marks, G. C. and Kozlowski, T. T.), pp. 43–78. Academic Press, London.

    Google Scholar 

  • Zak, B. 1976. Pure culture synthesis of Pacific madrone ectendomycorrhizae. Mycologia68: 362–369.

    Google Scholar 

  • Zak, B. and Bryan, W. C. 1963. Isolation of fungal symbionts from pine mycorrhizae. Forest Sci.9: 270–278.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Contribution No. 127, Laboratories of Plant Pathology and Mycology, Institute of Agriculture and Forestry, University of Tsukuba.

About this article

Cite this article

Yamada, A., Katsuya, K. Morphological classification of ectomycorrhizas ofPinus densiflora . Mycoscience 37, 145–155 (1996). https://doi.org/10.1007/BF02461339

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02461339

Key Words

Navigation