Abstract
Classical enzymology ignores the role which cellular membranes may play in regulating reaction kineticsin vivo. The correct description of cellular metabolic processes must derive from a mass balance equation for each reacting species. An elementary mathematical model, called the “continuous flow stirred tank reactor” in the chemical engineering literature, has been applied to Michaelis-Menten kinetics, substrate inhibition kinetics, and kinetics involving hydrogen ion as a byproduct. A number of remarkable phenomena, including multiple stationary states, threshold effects, temporal patterns, homeostatic regulation, amplification, and irreversible differentiation can result. Predictions of the model are in qualitative accord with experimental and theoretical studies of insolubilized enzymes, which are conventionally modeled by a more difficult mathematical formalism.
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Proceedings article from the Dissipative Structures Section of the Tenth Symposium on Biomathematics and Computer Science in the Life Sciences, University of Texas, Houston. March 29–31, 1973. Symposium Chairman: Stuart O. Zimmerman. Session Chairman and Proceedings Editors: Charles Walter and Hugo M. Martinez.
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Bunow, B. Enzyme kinetics in cells. Bltn Mathcal Biology 36, 157–169 (1974). https://doi.org/10.1007/BF02458600
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DOI: https://doi.org/10.1007/BF02458600