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Growth of an adherent continuous cell line entrapped in a peg/alginate matrix

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Biotechnology Techniques

Summary

CHO-K1 cells, an anchorage-dependent line, were entrapped in beads prepared from a Na alginate/polyethylene glycol mixture and grown, through successive passages, to an average maximum density of 4.5×107 viable cells/g of bead. Cell growth and viability was unaffected by repeated alginate re-solubilization and reformation of the gel beads through five passages.

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Author notes

  1. Gary J. Gates

    Present address: SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, 19406, King of Prussia, PA

Authors and Affiliations

  1. Department of Chemical Engineering, Lehigh University, Iacocca Hall 111 Research Drive, 18015, Bethlehem, PA

    Gary J. Gates & Janice A. Phillips

  2. BioProcessing Institute, Lehigh University, Iacocca Hall 111 Research Drive, 18015, Bethlehem, PA

    Gary J. Gates & Janice A. Phillips

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  1. Gary J. Gates
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  2. Janice A. Phillips
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Gates, G.J., Phillips, J.A. Growth of an adherent continuous cell line entrapped in a peg/alginate matrix. Biotechnol Tech 6, 517–522 (1992). https://doi.org/10.1007/BF02447824

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  • DOI: https://doi.org/10.1007/BF02447824

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