Summary
Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of polyurethane foam (PUF) and grew to a high cell density (1.1 × 108 cells/cm3 PUF and 4.2 × 107 cells/cm3 PUF, respectively) in a PUF-plates packed-bed culture system. This density of Vero cells was twice that obtained previously with a PUF-particles packed-bed culture system. A maximum cell density of 6.7 × 107 cells/cm3 culture vessel volume was obtained in a PUF-disc packed-bed culture of Vero cells. From the cell density of CHO-K1, growing in a monolayer on the surface of PUF and a petri dish, per bulk volume of PUF, we estimated that a surface area to volume ratio of PUF plates effective for cell growth was about 109 cm2/cm3.
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Matsushita, T., Hidaka, H., Kamihata, Ki. et al. High density culture of anchorage-dependent animal cells by polyurethane foam packed-bed culture systems. Appl Microbiol Biotechnol 35, 159–164 (1991). https://doi.org/10.1007/BF00184680
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DOI: https://doi.org/10.1007/BF00184680