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Immunoblotting of mite aeroallergens collected with an indoor Burkard air sampler

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Abstract

We examined the kinetics of airborne levels of mite allergen particles in a house by combined use of an indoor Burkard air sampler and immunoblotting. Airborne mite allergens collected on the Burkard sampling tape were transferred onto a nitrocellulose membrane, reacted with mouse monoclonal anti-mite allergen (Der pI) antibody, then treated with alkaline phosphatase conjugated anti-mouse IgG. Finally, the blotted allergen on the membrane was reacted with BCIP/NBT phosphatase, and purple spots visible by the naked eye were produced. The shape of the spots was observed under a microscope, and the spot area was measured by an image processor. This technique might be useful for analyzing the behavior of airborne allergen particles in indoor environments.

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Authors and Affiliations

  1. department of Epidemiology, National Institute of Health, Toyama 1-23-1 Shinjuku-ku, 162, Tokyo, Japan

    Masahiro Sakaguchi & Sakae Inouye

  2. The Yamagata Prefectural Institute of Public Health, Yamagata, Japan

    Yuichi Takahashi & Susumu Kutagiri

  3. Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Sagamihara, Japan

    Hiroshi Yasueda

Authors
  1. Masahiro Sakaguchi
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  2. Sakae Inouye
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  3. Yuichi Takahashi
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  4. Susumu Kutagiri
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  5. Hiroshi Yasueda
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Sakaguchi, M., Inouye, S., Takahashi, Y. et al. Immunoblotting of mite aeroallergens collected with an indoor Burkard air sampler. Aerobiologia 11, 265–268 (1995). https://doi.org/10.1007/BF02447207

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  • DOI: https://doi.org/10.1007/BF02447207

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