Skip to main content
SpringerLink
Log in

Modulation of interleukin-2-induced proliferation and nonspecific cytotoxicity of rat lymphocytes by phorbol myristate acetate and staurosporine

  • Biophysics and Biochemistry
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

It is shown that a 19-h pretreatment of rat splenocytes with 1 μM phorbol 12-myristate 13-acetate followed by a 42-h incubation with human recombinant interleukin-2 inhibits nonspecific cytotoxicity of these cells toward the target YAC-1 cells. By contrast, proliferation of splenocytes and thymocytes incubated under the same conditons increases considerably. The inhibitor of protein kinase C staurosporine (0.1 μM) significantly decreases nonspecific cytotoxicity of splenocytes after a 20-min incubation, while in a dose of 0.01 μM it stimulates lytic activity of splenocytes and thymocytes following a 3-day incubation with interleukin-2 in the presence of the inhibitor. Cell proliferation under these conditions is markedly decreased.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. I. A. Bolotnikov and A. G. Anisimov,Immunologiya, No. 2, 17–23 (1994).

    Google Scholar 

  2. M. P. Potapnev, D. V. Pechkovskii, T. S. Garbuzenko,et al., Ibid., No. 6, 30–32, (1994).

    Google Scholar 

  3. A. M. Sorokin, S. B. Cheknev, and B. P. KuznetsovIbid., No. 1, 17–20 (1991).

    Google Scholar 

  4. Z. K. Ballas and W. Rasmussen,J. Immunol.,139, No. 10, 3542–3549 (1987).

    PubMed  CAS  Google Scholar 

  5. Z. K. Ballas and W. RasmussenIbid.,144, No. 1, 386–395 (1990).

    PubMed  CAS  Google Scholar 

  6. I. A. Bolotnikov and A. G. Anisimov,Int. J. Immunorehab., Suppl. 1, 70 (1994).

  7. W. L. Farrar, D. K. Ferris, and A. Harrel-Bellan, in:Advanced in Immunopharmacology, Oxford (1989), pp. 205–209.

  8. S. J. LeGrue,Lymphokine Res.,7, No. 2, 187–200 (1988).

    PubMed  CAS  Google Scholar 

  9. M. C. Mingari, P. Varese, C. Bottino,et al., Ric. Clin. Lab.,18, No. 1, 67–73 (1988).

    Article  PubMed  CAS  Google Scholar 

  10. Y. Nishizuka,Nature,334, No. 6184, 661–665 (1988).

    Article  PubMed  CAS  Google Scholar 

  11. A. D. G. Procopio, R. Paolini, A. Gismondi,et al., Cell. Immunol.,118, No. 2, 470–481 (1989).

    Article  PubMed  CAS  Google Scholar 

  12. J. P. Secrist, L. Karnitz, and R. T. Abraham,J. Biol. Chem.,266, No. 19, 12135–12139 (1991).

    PubMed  CAS  Google Scholar 

  13. H. Stotter, M. C. Custer, E. S. Bolton,et al., J. Immunol.,146, No. 1, 150–155 (1991).

    PubMed  CAS  Google Scholar 

  14. T. Tamaoki, H. Nomoto, I. Takahashi,et al., Biochem. Biophys. Res. Commun.,135, No. 2, 397–402 (1986).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Petrozavodsk State University, Petrozavodsk, USSR

    A. G. Anisimov & I. A. Bolotnikov

Authors
  1. A. G. Anisimov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. A. Bolotnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 10, pp. 394–398, October, 1996

Rights and permissions

Reprints and permissions

About this article

Cite this article

Anisimov, A.G., Bolotnikov, I.A. Modulation of interleukin-2-induced proliferation and nonspecific cytotoxicity of rat lymphocytes by phorbol myristate acetate and staurosporine. Bull Exp Biol Med 122, 994–997 (1996). https://doi.org/10.1007/BF02447017

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02447017

Key Words

Search

Navigation