Abstract
A toxicological study of a preparation of king crab collagenase designed for local treatment of wounds shows that daily hypodermal administration of various concentrations of preparation (1.5 to 15 mg/ml) over 5 days does not affect erythrocyte morphology or hemoglobin content in laboratory animals (rats and rabbits). Systemic enzyme administration does not alter the histological structure of the kidneys (kidney is the target organ for enzyme) but leads to dose-dependent reversible degeneration of rat liver tissues. The milder effects of the preparation in comparison with a comparable dose of the widely used chymopsin affirm that this collagenase preparation is suitable for external use.
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References
A. A. Adamyan, S. P. Glyantsev, I. Yu. Sakharov, and T. V. Savvina,Byull. Eksp. Biol. Med.,114, No 12, 660–663 (1992).
V. A. Burobin, in:Methods of Investigation of Some Enzymes in a Clinical Setting [in Russian], Moscow (1967), pp. 28–38.
S. P. Glyantsev,Development of Modern Enzyme-Containing Dressings and Improving Their Application to Complex Purulent Wound Treatment [in Russian], M. D. dissertation, Moscow (1993).
Laboratory Methods in Clinical Investigations [in Russian], Ed. V. V. Men'shikov, Moscow (1987).
G. A. Merkulov,Handbook of Pathological Histology Technique [in Russian], Leningrad (1961).
I. Yu. Sakharov, S. P. Glyantsev, F. E. Litvin, and V. F. Gordeev,Vopr. Med. Khim., No 3, 18–20 (1994).
I. Yu. Sakharov, F. E. Litvin, A. A. Artyukov, and N. N. Kofanova,Biokhimiya,53, No 11, 1844–1849 (1988).
I. Yu. Sakharov, B. V. Shekhonin, S. P. Glyantsev, and F. E. Litvin,Byull. Eksp. Biol. Med.,116, No 9, 267–270 (1993).
A. I. Strukov and V. V. Serov,Pathological Anatomy [in Russian], Moscow (1993).
I. Mandl, G. D. MacLennan, and E. L. Howes,J. Clin. Invest.,32, 1323–1329 (1953).
I. Yu. Sakharov, S. P. Glyantsev, F. E. Litvin, and T. V. Savvina,Arch. Dermatol. Res.,285, No 1-2, 32–35 (1993).
H. Tabor and A. H. Mehler, in:Methods in Enzymology, Eds. S. P. Colavicket al., Vol. 2, New York (1955), p. 228.
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, No 4, pp. 390–393, April, 1995
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Glyantsev, S.P., Savvina, T.V., Litvin, F.E. et al. Toxicological study of king crab collagenase. Bull Exp Biol Med 119, 377–380 (1995). https://doi.org/10.1007/BF02445898
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DOI: https://doi.org/10.1007/BF02445898