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Relationship between intracellular cAMP level in intact splenocytes and population composition of cell suspension and cycloxygenase activity

  • Genetics
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Abstract

A maximum increment in cAMP was detected by radioimmunoassay on the 30th min ofin vitro incubation of mouse splenocytes in nonfractionated suspension, while in fractionated T or B cells cultures the level of cAMP was the lowest. Removal of A cells from suspension or blocking of endogenous eicosanoid production alters the cAMP curve approximating it to distribution in fractionated lymphocytes. Therefore, splenic macrophages are the main regulators of intracellular cAMP level in intact splenocyte suspension, realizing their effect through cycloxygenase products.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of Russian Academy of Sciences, Perm

    S. V. Shirshev

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  1. S. V. Shirshev
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 6, pp. 666–669, June, 1998

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Shirshev, S.V. Relationship between intracellular cAMP level in intact splenocytes and population composition of cell suspension and cycloxygenase activity. Bull Exp Biol Med 125, 591–593 (1998). https://doi.org/10.1007/BF02445249

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  • DOI: https://doi.org/10.1007/BF02445249

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