Abstract
Rhizobium meliloti 300hl3 reached the stationary phase of growth very quickly and had an early death when 8.6 μM parathion was added to the growth medium at the start of the culture. Cells also changed their quantitative composition: total carbohydrate content and poly-β-hydroxybutyrate accumulation diminished, proteins and phospholipids of cellular membranes increased, and some alterations in the proportion of membrane fatty acids were noticed.
Similar content being viewed by others
References
Ames, G. F. 1968. Lipids ofSalmonella typhimurium andEscherichia coli: structure and metabolism. —J. Bacteriol.95: 833–843.
Bligh, E. G. andDyer, W. J. 1959. A rapid method of total lipid extraction and purification. —Can. J. Biochem. Physiol.37: 911–917.
Cook, A. M., Grossenbacher, H. andHotter, R. 1983. Isolation and cultivation of microbes with degradative potential. —Experientia39: 1191–1198.
Dazzo, F. B. andTruchet, G. L. 1983. Interactions of lectins and their saccharide receptors in theRhizobium-legume symbiosis. —J. Membr. Biol.73: 1–16.
Dodge, J. T. andPhillips, G. B. 1967. Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells. —J. Lipid Res.8: 667–675.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. andSmith, F. 1956. Colorimetric method for determination of sugars and related substances. —Anal. Chem.28: 350–356.
Evans Jr, D. J. 1969. Membrane adenosine tripbosphatase ofEscherichia coli: activation by calcium ion and inhibition by monovalent cations. —J. Bacteriol.100: 914–922.
Gerson, T. andPatel, J. J. 1975. Neutral lipids and phospholipids of free-living and bacteroid forms of two strains ofRhizobium infective onLotus pedunculatus. —Appl. Microbiol.30: 193–198.
Kulkarni, J. H., Sardeshpande, J. S. andBagyaraj, D. J. 1974. Effect of four soil-applied insecticides on symbiosis ofRhizobium sp. withArachis hypogaea Linn. —Plant Soil40: 169–172.
Lin, S.-C., Funke, B. R. andSchulz, J. T. 1972. Effects of some organophosphate and carbamate insecticides on nitrification and legume growth. —Plant Soil37: 489–496.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. —J. Biol. Chem.193: 265–275.
MacRae, C. andCelo, J. S. 1974. The effects of organo-phosphorus pesticides on the respiration ofAzotobacter vinelandii. —Soil Biol. Biochem.6: 109–111.
Mallik, M. A. B. andTesfai, K. 1983. Compatibility ofRhizobium japonicum with commercial pesticides in vitro. —Bull. Environ. Contam. Toxicol.31: 432–437.
Rosas, S. B., Secco, M. C. andGhittoni, N. E. 1980. Effects of pesticides on the fatty acid and phospholipid composition ofEscherichia coli. —Appl. Environ. Microbiol.40: 231–234.
Sekar, T. andBalasubramanian, A. 1979. Influence of a carbamate pesticide on growth, respiration (14C)-carbon metabolism and symbiosis of aRhizobium sp. —Plant Soil51: 355–361.
Sethunathan, N. 1973. Degradation of parathion in flooded acid soils. —J. Agric. Food Chem.21:602–604.
Zevenhuizen, L. P. T. M. 1981. Cellular glycogen, β-1, 2-glucan, poly-β-hydroxybutyric acid and extracellular polysaccharides in fast-growing species ofRhizobium. —Antonie van Leeuwenhoek47:481–497.
Zevenhuizen, L. P. T. M. andEbbink, A. G. 1974. Interrelations between glycogen, poly-β-hydroxybutyric acid and lipids during accumulation and subsequent utilization in aPseudomonas. —Antonie van Leeuwenhoek40: 103–120.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Carranza de Storani, M.M., Rosas, S.B. & Ghittoni, N.E. Effect of parathion on growth, polysaccharides, lipids and proteins ofRhizobium meliloti . Antonie van Leeuwenhoek 51, 249–254 (1985). https://doi.org/10.1007/BF02439934
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02439934