Skip to main content
Log in

Effect of parathion on growth, polysaccharides, lipids and proteins ofRhizobium meliloti

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Rhizobium meliloti 300hl3 reached the stationary phase of growth very quickly and had an early death when 8.6 μM parathion was added to the growth medium at the start of the culture. Cells also changed their quantitative composition: total carbohydrate content and poly-β-hydroxybutyrate accumulation diminished, proteins and phospholipids of cellular membranes increased, and some alterations in the proportion of membrane fatty acids were noticed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ames, G. F. 1968. Lipids ofSalmonella typhimurium andEscherichia coli: structure and metabolism. —J. Bacteriol.95: 833–843.

    PubMed  CAS  Google Scholar 

  • Bligh, E. G. andDyer, W. J. 1959. A rapid method of total lipid extraction and purification. —Can. J. Biochem. Physiol.37: 911–917.

    PubMed  CAS  Google Scholar 

  • Cook, A. M., Grossenbacher, H. andHotter, R. 1983. Isolation and cultivation of microbes with degradative potential. —Experientia39: 1191–1198.

    Article  PubMed  CAS  Google Scholar 

  • Dazzo, F. B. andTruchet, G. L. 1983. Interactions of lectins and their saccharide receptors in theRhizobium-legume symbiosis. —J. Membr. Biol.73: 1–16.

    Article  CAS  Google Scholar 

  • Dodge, J. T. andPhillips, G. B. 1967. Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells. —J. Lipid Res.8: 667–675.

    PubMed  CAS  Google Scholar 

  • Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. andSmith, F. 1956. Colorimetric method for determination of sugars and related substances. —Anal. Chem.28: 350–356.

    Article  CAS  Google Scholar 

  • Evans Jr, D. J. 1969. Membrane adenosine tripbosphatase ofEscherichia coli: activation by calcium ion and inhibition by monovalent cations. —J. Bacteriol.100: 914–922.

    PubMed  CAS  Google Scholar 

  • Gerson, T. andPatel, J. J. 1975. Neutral lipids and phospholipids of free-living and bacteroid forms of two strains ofRhizobium infective onLotus pedunculatus. —Appl. Microbiol.30: 193–198.

    PubMed  CAS  Google Scholar 

  • Kulkarni, J. H., Sardeshpande, J. S. andBagyaraj, D. J. 1974. Effect of four soil-applied insecticides on symbiosis ofRhizobium sp. withArachis hypogaea Linn. —Plant Soil40: 169–172.

    Article  CAS  Google Scholar 

  • Lin, S.-C., Funke, B. R. andSchulz, J. T. 1972. Effects of some organophosphate and carbamate insecticides on nitrification and legume growth. —Plant Soil37: 489–496.

    Article  CAS  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L. andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. —J. Biol. Chem.193: 265–275.

    PubMed  CAS  Google Scholar 

  • MacRae, C. andCelo, J. S. 1974. The effects of organo-phosphorus pesticides on the respiration ofAzotobacter vinelandii. —Soil Biol. Biochem.6: 109–111.

    Article  CAS  Google Scholar 

  • Mallik, M. A. B. andTesfai, K. 1983. Compatibility ofRhizobium japonicum with commercial pesticides in vitro. —Bull. Environ. Contam. Toxicol.31: 432–437.

    Article  CAS  Google Scholar 

  • Rosas, S. B., Secco, M. C. andGhittoni, N. E. 1980. Effects of pesticides on the fatty acid and phospholipid composition ofEscherichia coli. —Appl. Environ. Microbiol.40: 231–234.

    PubMed  CAS  Google Scholar 

  • Sekar, T. andBalasubramanian, A. 1979. Influence of a carbamate pesticide on growth, respiration (14C)-carbon metabolism and symbiosis of aRhizobium sp. —Plant Soil51: 355–361.

    Article  CAS  Google Scholar 

  • Sethunathan, N. 1973. Degradation of parathion in flooded acid soils. —J. Agric. Food Chem.21:602–604.

    Article  PubMed  CAS  Google Scholar 

  • Zevenhuizen, L. P. T. M. 1981. Cellular glycogen, β-1, 2-glucan, poly-β-hydroxybutyric acid and extracellular polysaccharides in fast-growing species ofRhizobium. —Antonie van Leeuwenhoek47:481–497.

    Article  PubMed  CAS  Google Scholar 

  • Zevenhuizen, L. P. T. M. andEbbink, A. G. 1974. Interrelations between glycogen, poly-β-hydroxybutyric acid and lipids during accumulation and subsequent utilization in aPseudomonas. —Antonie van Leeuwenhoek40: 103–120.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carranza de Storani, M.M., Rosas, S.B. & Ghittoni, N.E. Effect of parathion on growth, polysaccharides, lipids and proteins ofRhizobium meliloti . Antonie van Leeuwenhoek 51, 249–254 (1985). https://doi.org/10.1007/BF02439934

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02439934

Keywords

Navigation