Summary
Cell-free synthesis of galactose enzymes was directed by heteroduplex DNA carrying an amber mutation in thegal T gene on one DNA strand, the wildtype configuration on the complementary strand. Comparison with the corresponding homoduplex DNA preparations shows that the presence of the wildtype base sequence in the l-strand is necessary and sufficient for the synthesis of active enzyme. Single stranded DNA of either configuration is inactive in this system.
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Communicated by H. G. Wittmann
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Wetekam, W. Identification of template strand in heteroduplex DNA directing cell-free enzyme synthesis. Molec. Gen. Genet. 118, 57–60 (1972). https://doi.org/10.1007/BF02428333
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DOI: https://doi.org/10.1007/BF02428333