Summary
Upon induction, the three galactose enzymes appear in the order epimerase, transferase, kinase. Introduction of an amber mutation into the transferase gene seems to shorten the time interval between addition of inducer and the appearance of kinase. The time of appearance of epimerase is not influenced by amber mutations in the transferase gene.
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Alpers, D. H., and G. M. Tomkins: The order of induction and deinduction of the enzymes of the lactose operon in E. coli. Proc. nat. Acad. Sci. (Wash.) 53, 797–803 (1965).
—: Sequential transcription of the genes of the lactose operon and its regulation by protein synthesis. J. biol. Chem. 241, 4434–4443 (1966).
Ashworth, J. M., H. L. Kornberg, and D. L. Nothmann: Location of the structural gene for citrate synthase on the chromosome of Escherichia coli K 12. J. molec. Biol. 11, 654–657 (1965).
Berberich, M. A., P. Venetianer, and R. F. Goldberger: Alternative modes of derepresion of the histidine operon observed in Salmonella typhimurium. J. biol. Chem. 241, 4426–4433 (1966).
Buttin, G.: Mécanismes régulateurs dans la biosynthèse des enzymes du métabolisme du galactose chez Escherichia coli K 12. II. Le déterminisme génétique de la régulation. J. molec. Biol. 7, 183–205 (1963).
Dennert, G., R. Hertel, G. Deppe, and U. Henning: Action of an amber suppressor gene carried by phage φ80. Z. Vererbungsl. 97, 243–254 (1965).
Goldberger, R. F., and M. A. Berberich: Sequential repression and derepression of the enzymes for histidine biosynthesis in Salmonella typhimurium. Proc. nat. Acad. Sci. (Wash.) 54, 279–286 (1965).
Imamoto, F., J. Ito, and C. Yanofsky: Polarity in the tryptophan operon of E. coli. Cold Spr. Harb. Symp. quant. Biol. 31, 235–249 (1966).
Jordan, E., and H. Saedler: Polarity of amber mutations and suppressed amber mutations in the galactose operon of E. coli. In press.
Kepes, A.: Sequential transcription and translation in the lactose operon of Escherichia coli. Biochem. biophys. Acta (Amst.) 138, 107–123 (1967).
Leive, L., and V. Kollin: Synthesis utilization and degradation of lactose operon mRNA in Escherichia coli. J. molec. Biol. 24, 247–259 (1967).
Lengeler, J.: Untersuchungen zum Glukose-Effekt bei der Synthese der Galaktose-Enzyme von Escherichia coli. Z. Vererbungsl. 98, 203–229 (1966).
Lennox, E. S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955).
Martin, R. G., D. F. Silbert, D. W. E. Smith, and H. J. Whitfield: Polarity in the histidine operon. J. molec. Biol. 21, 357–369 (1966).
Pfeifer, D., and R. Oellermann: Mapping of gal-mutants by transducing λdg carrying deletions of the gal-region. Molec. Gen. Genetics 99, 248–252 (1967).
Saedler, H., and P. Starlinger: O oMutations in the galactose operon of E. coli. II. Physiological characterization. Molec. Gen. Genetics 100, 190–202 (1967).
Weber, E.: Grundriß der biologischen Statistik, 5. Aufl. Jena: VEB Gustav Fischer 1964.
Yanofsky, C., and J. Ito: Nonsense codons and polarity in the tryptophan operon. J. molec. Biol. 21, 313–334 (1966).
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Michaelis, G., Starlinger, P. Sequential appearance of the galactose enzymes in E. coli . Molec. Gen. Genetics 100, 210–215 (1967). https://doi.org/10.1007/BF00333607
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DOI: https://doi.org/10.1007/BF00333607