Summary
An amplified sequence of mitochondrial DNA from a ragged (rgd) mutant ofAspergillus amstelodami has been shown to exist in multimeric circular form, suggesting that it is excised from the genome and can exist independently of it. This sequence has replicative (ARS) activity inSaccharomyces cerevisiae, and a subfragment responsible for this activity has been identified and sequenced. A homologous sequence fromAspergillus nidulans mtDNA also has ARS activity inS. cerevisiae. BothA. amstelodami andA. nidulans ARS elements have been incorporated into the integrative transformation vector pDJBI and the derived vectors used to transformA. nidulans. Inclusion of theA. nidulans ARS element enhanced the transformation frequency 5-fold relative to pDJBI. No increase in transformation frequency was evident with the ARS element fromA. amstelodami. The stability of transformants was variable but in comparison to pDJBI, ARS-containing plasmids were mitotically unstable inA. nidulans. Although plasmid DNAs could be rescued inEscherichia coli from undigested transformant DNA, no freely replicating plasmids were detected by Southern hybridisation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bach ML, Lacroute F, Botstein D (1979) Proc Natl Acad Sci USA 76:386–390
Ballance DJ, Buxton FP, Turner G (1983) Biochem Biophys Res Commun 112:284–289
Ballance DJ, Turner G (1985) Gene 36:321–331
Barnes DE, MacDonald DW (1986) Curr Genet 10:767–775
Beach D, Piper M, Nurse P (1982) Mol Gen Genet 187:326–329
Beggs JD (1978) Nature 275:104–109
Bennett PM, Richmond MH (1976) J Bacteriol 126:1–6
Bernardi G (1982) Trends Biochem Sci 7:404–408
Botstein D, Falco SC, Stewart SE, Stinchcomb DT, Struhl K, Davis RW (1979) Gene 8:17–24
Broach JR, Li Y-Y, Feldman J, Jayaram M, Abraham J, Nasmyth KA, Hicks JB (1983) Cold Spring Harbor Symp Quant Biol 45:1165–1173
Bull JH, Wootton JD (1984) Nature 310:701–704
Buxton FP, Radford A (1983) Mol Gen Genet 190:403–405
Casadaban MJ, Cohen SN (1980) J Mol Biol 138:179–207
Case ME (1982) In: Hollaender A, Demoss RD, Koninsky J, Savage D, Wolfe RS (eds) Genetic engineering of microorganisms for chemicals. Plenum Press, New York, pp 87–100
Celniker SE, Sweder K, Srienc F, Bailey JE, Campbell JL (1984) Mol Cell Biol 4:2455–2466
Conely EC, Saunders JR (1984) Mol Gen Genet 194:211–218
Crews S, Ojala D, Posakony J, Nishiguchi J, Attardi G (1979) Nature 277:192–198
Cummings DJ, Belcour L, Grandchamp C (1979) Mol Gen Genet 171:239–249
De Zamaroczy M, Faugeron-fonty G, Baldacci G, Goursot R, Bernardi G (1984) Gene 32:439–457
Grant DM, Lambowitz AM, Rambosek JA, Kinsey JA (1984) Mol Cell Biol 4:2041–2051
Hahn U, Lazarus CM, Lunsdorf H, Kuntzel H (1979) Cell 17:191–200
Hanahan D (1983) J Mol Biol 166:557–580
Heeswijk R van (1986) Carlsberg Res Commun 51:433–443
Holmes DS, Quigley M (1981) Anal Biochem 114:193–197
Johnstone IL, Hughes SG, Clutterbuck AJ (1985) EMBO J 4:1307–1311
Kearsey S (1984) Cell 37:299–307
Lazarus CM, Earl AJ, Turner G, Kuntzel H (1980) Eur J Biochem 106:633–641
Lazarus CM, Kuntzel H (1981) Curr Genet 4:99–107
Lazdins IB, Cummings DJ (1982) Curr Genet 6:173–178
Lazowska J, Slonimski PP (1976) Mol Gen Genet 146:61–78
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (A laboratory manual)
Marinus MG, Konrad EB (1976) Mol Gen Genet 149:273–277
Maundrell K, Wright APH, Piper M, Shall S (1985) Nucleic Acids Res 13:3711–3722
Messing J (1983) Methods Enzymol 101:20–78
Morris NR (1978) J Gen Microbiol 106:387–389
Netzker R, Kochel HG, Basak N, Kuntzel H (1982) Nucleic Acids Res 10:4783–4794
Paietta J, Marzluf G (1985) Curr Genet 9:383–388
Palmer LM, Cove DJ (1975) Mol Gen Genet 138:243–255
Perrot M, Barreau C, Begueret J (1987) Mol Cell Biol 7:1725–1730
Roth GE, Blanton HM, Hafer LJ, Zakian VA (1983) Mol Cell Biol 3:1898–1908
Rowlands RT, Turner G (1973) Mol Gen Genet 126:201–206
Sakaguchi J, Yamamoto M (1982) Proc Natl Acad Sci USA 79:7819–7823
Skatrud PL, Queener SW, Carr LG, Fisher DL (1987) Curr Genet 12:337–348
Stinchcomb DT, Thomas M, Kelly S, Selber ER, Davis RW (1980) Proc Natl Acad Sci USA 77:4559–4563
Stohl LL, Lambowitz AM (1983) Proc Natl Acad Sci USA 80:1058–1062
Tikhomirova LP, Ikonomova RN, Kuznetsova EN (1986) Curr Genet 10:741–747
Tilburn J, Scazzacchio C, Taylor GG, Zabicky-zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221
Tudzynski P, Esser K (1985) In: Bennet JW, Lasure LL (eds) Gene manipulation in fungi. Academic Press, New York, pp 403–416
Turner G, Ballance DJ, Ward M, Beri RK (1985) In: Timberlake WE (ed) Molecular genetics of filamentous fungi. UCLA Symposia on Molecular and Cellular Biology, new series, vol 34. Liss, New York
Viera J, Messing J (1982) Gene 19:259–268
Williamson DH (1985) Yeast 1:1–14
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beri, R.K., Lewis, E.L. & Turner, G. Behaviour of a replicating mitochondrial DNA sequence fromAspergillus amstelodami inSaccharomyces cerevisiae andAspergillus nidulans . Curr Genet 13, 479–486 (1988). https://doi.org/10.1007/BF02427753
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02427753