Summary
The wild type nematode,Caenorhabditis elegans, moves in a sinusoidal wave pattern and leaves sinusoidal paths behind it on a bacterial lawn. The nematode crawls on its side on a special cuticular tread that extends straight down the length of its body. Wild type worms also have rows of musculature and a ventral nerve cord that extend straight down the body. Roller mutants rotate around their long axis as they crawl and move in circular paths. Three roller mutants have been studied. Two mutants are left rollers and one is a right roller. The left rollers have left-handed helical treads, body musculatures, and ventral nerve cords whereas these structures are right-handed helices in the right roller. Double mutants constructed from roller mutants and long mutants indicate that long rollers have helices of the same pitch as normal length rollers. Double mutants constructed from rollers and dumpy mutants that are short and fat indicate dumpy phenotype is epistatic to roller. Double mutants constructed from rollers and blister mutants that have cuticular swelling indicate roller phenotype is epistatic to blister. The results suggest that the roller phenotypes are due to cuticular lesions. Rollers can chemotaxe up a gradient of an attractant by turning off their body muscle movement and continuing their head movements.
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Bird, A.F., Deutsch, K.: The structure of the cuticle ofAscaris lumbricoides var. Suis. Parasitology47, 319–328 (1957)
Boycott, A.E., Diver, C., Garstang, S.L., Turner, F.M.: The inheritance of sinistrality inLimnaea peregra. Phil. Trans. B219, 51–131 (1930)
Brenner, S.: The genetics ofCaenorhabditis elegans. Genetics77, 71–94 (1974)
Dusenbery, D.B.: Analysis of chemotaxis in the nematodeCaenorhabditis elegans by countercurrent separation. J. exp. Zool.188, 41–48 (1974)
Hirsh, D., Vanderslice, R.: Temperature sensitive mutants ofCaenorhabditis elegans. Develop. Biol.49, 220–235 (1976)
Humason, G.G., Animal tissue techniques. San Fransisco: W.H. Freeman and Co. 1967
Josse, J., Harrington, W.F.: Role of pyrrolidine residues in the structure and stabilization of collagen. J. molec. Biol.9, 269–287 (1964)
Ward, S.: Chemotaxis by the nematodeCaenorhabditis elegans: Identification of attractants and analyses of the response by the use of mutants. Proc. nat. Acad. Sci. (Wash.)70, 817 (1973)
Ward, S., Thomson, N., White, J.G., Brenner, S.: Electron microscopical reconstruction of the anterior sensory anatomy of the nematodeCaenorhabditis elegans. J. comp. Neurol.160, 313–338 (1975)
Ware, R.W., Clark, D., Crossland, K., Russell, R.L.: The nerve ring of the nematodeCaenorhabditis elegans: Sensory input and motor input. J. comp. Neurol.162, 71–110 (1975)
Wood W.: Genetic control of bacteriophage T4 morphogenesis. In: Genetic mechanisms of development (F. Ruddle, ed.), pp. 29–46. New York: Academic Press 1973
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Communicated by L. Lerman
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Higgins, B.J., Hirsh, D. Roller mutants of the nematodeCaenorhabditis elegans . Molec. Gen. Genet. 150, 63–72 (1977). https://doi.org/10.1007/BF02425326
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DOI: https://doi.org/10.1007/BF02425326