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Excision-repair in mutants ofEscherichia coli deficient in DNA polymerase I and/or its associated 5′→3′ exonuclease

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Summary

The ultraviolet (UV) sensitivity ofEscherichia coli mutants deficient in the 5′→3′ exonuclease activity of DNA polymerase I is intermediate between that ofpol + strains and mutants which are deficient in the polymerizing activity of pol I (polA1). LikepolA1 mutants, the 5′-exonuclease deficient mutants exhibit increased UV-induced DNA degradation and increased repair synthesis compared to apol + strain, although the increase is not as great as inpolA1 or in the conditionally lethal mutant BT4113ts deficient inboth polymerase I activities. When dimer excision was measured at UV doses low enough to avoid interference from extensive DNA degradation, all three classes of polymerase I deficient mutants were found to remove dimers efficiently from their DNA. We conclude that enzymes alternative to polymerase I can operate in both the excision and resynthesis steps of excision repair and that substitution for either of the polymerase I functions results in longer patches of repair. A model is proposed detailing the possible events in the alternative pathways.

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Authors and Affiliations

  1. Department of Biological Sciences, Stanford University, 94305, Stanford, California

    Priscilla Cooper

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  1. Priscilla Cooper
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Communicated by E. Witkin

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Cooper, P. Excision-repair in mutants ofEscherichia coli deficient in DNA polymerase I and/or its associated 5′→3′ exonuclease. Molec. Gen. Genet. 150, 1–12 (1977). https://doi.org/10.1007/BF02425319

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  • DOI: https://doi.org/10.1007/BF02425319

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