Abstract
The sequence of a feline class I pseudogene and its comparison with class I genes from other species is presented. The gene isolated is a pseudogene because of the presence of four stop codons and two frame shift mutations in the first-and second-domain encoding exons, as well as a mutation in a splice acceptor site in the third intron. By sequence comparison with the other class I sequences determined to date, theFLA pseudogene is most closely related to theHLA-A locus products (88% nucleotide identity).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breathnach, R. and Chambon, P. Organisation and expression of eukaryotic split genes for proteins.Annu Rev Biochem 50:349–383, 1981
Colbert, E. H.:Evolution of the Vertebrates, John Wiley, New York, 1969.
Frischauf, A. M., Lehrach, H., Poustka, A., and Murray, N.: Lambda replacement vectors carrying polylinker sequences.J Mol Biol 170: 827–842, 1983
Güssow, D., Rein, R. S., Meijer, I., de Hoog, W., Seemann, G. H. A., Hochstenbach, F. M., and Ploegh, H. L.: Isolation, expression, and the primary structure ofHLA-Cwl andHLA-Cw2 genes: evolutionary aspects.Immunogenetics 25: 313–322, 1987
Hayashida, H. and Miyata, T.: Unusual evolutionary conservation and frequent DNA segment exchange in class I genes of the major histocompatibility complex.Proc Natl Acad Sci USA 80; 2671–2675, 1983
Klein, J.: Generation of diversity at MHC loci: implications for T cell receptor repertoirs.In M. Fougereau and J. Dausset (eds.):Immunology 80, pp. 239–253, Academic Press, London, 1980
Klein, J.:Natural History of the Major Histocompatibility Complex, John Wiley, New York, 1986
Klein, J.: Origin of major histocompatibility complex polymorphism: the trans-species hypothesis.Hum Immunol 19: 155–162, 1987
Koller, B. H. and Orr, H. T.: Cloning and complete sequence of an HLA-A2 gene: analysis of two HLA-A alleles at the nucleotide level.J Immunol 134: 2727–2733, 1985
Kottmann, A. H., Seemann, G. H. A., Güssow, H. D., and Roos, M. H.: DNA sequence of the coding region of theHLA-B44 gene.Immunogenetics 23: 396–400, 1986
Kvist, S., Roberts, L., and Dobberstein, B.: Mouse histocompatibility genes: structure and organisation of a Kd gene.EMBO J 2: 245–254, 1983
Maniatis, T., Fritsch, E. F., and Sambrook, J.:Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, 1982
Marche, P. N., Tykocinski, M. L., Max, E. E., and Kindt, T. J.: Structure of a functional rabbit class I MHC gene: similarity to human class I genes.Immunogenetics 21: 71–82, 1985
McCarthy, J. M. and Grant, C. K.: Cellular immune response in the blood of cats is restricted to autochtonous feline sarcoma virus transformed cells.Int J Cancer 31: 627–631, 1983
Miyata, T., Yasunaga, T., and Nishida, T.: Nucleotide sequence divergence and functional constraint in mRNA evolution.Proc Natl Acad Sci USA 77: 7328–7332, (1980)
Moore, K. W., Sher, B. T., Sun, Y. H., Eakle, K. A., and Hood, L.: DNA sequence of a gene encoding a Balb/c mouse Ld transplantation antigen.Science 215: 679–682, 1982
Neefjes, J. J., Hensen, E. J., de Kroon, T. I. P., and Ploegh, H. L.: A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.Immunogenetics 23: 341–347, 1986
Parham, P., Lomen, C. E., Lawlor, D. A., Ways, J. P., Holmes, N., Coppin, H. L., Salter, R. D., Wan, A. M., and Ennis, P. D.: Nature of polymorphism in HLA-A,-B, and-C molecules.Proc Natl Acad Sci USA 85: 4005–4009, 1988
Pollack, M. S., Mastrota, F., Chin-Louie, J., Mooney, S., and Hayes, A.: Preliminary studies of the feline histocompatibility system.Immunogenetics 16: 339–347, 1982
Sackstein, R., Colten, H. R., and Woods, D. E.: Phylogenetic conservation of a class III major histocompatibility complex antigen, factor B. Isolation and nucleotide sequencing of mouse factor B cDNA clones.J Biol Chem 258: 14693–14697, 1983
Seemann, G. H. A., Rein, R. S., Brown, C. S., and Ploegh, H. L.: Gene conversion-like mechanisms may generate polymorphism in human class I genes.EMBO J 5: 547–552, 1986
Sher, B. T., Nairn, R., Coligan, J. E., and Hood, L. E.: DNA sequence of the mouse H-2Dd transplantation antigen gene.Proc Natl Acad Sci USA 82: 1175–1179, 1985
Sood, A. K., Pereira, D., and Weissman, S. M.: Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.Proc Natl Acad Sci USA 78: 616–620, 1981
Sood, A. K., Pan, J., Biro, P. A., Pereira, D., Srivastava, R., Reddy, V. B., Duceman, B. W., and Weissman, S. M.: Structure and polymorphism of class I MHC antigen mRNA.Immunogenetics 22: 101–121, 1985
Stiff, M. I. and Olsen, R. G.: Feline one-way mixed lymphocyte reaction.Vet Immunol Immunopathol 7: 1–9, 1984
Strachan, T., Sodoyer, R., Damotte, M., and Jordan, B. R.: Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes.EMBO J 3: 887–894, 1984
Thenius, E.:Die Evolution der Säugetiere, (Gustav Fischer Verlag, Stuttgart, 1979
Tykocinski, M. L., Marche, P. N., Max, E. E., and Kindt, T. J.: Rabbit class I MHC genes: cDNA clones define full-length transcripts of an expressed gene and a putative pseudogene.J Immunol 133: 2261–2269, 1984
Verhoeven, A. J. M., Roos, M. H., and Ploegh, H. L.: A feline class II α gene with striking similarity to theHLA-DPA pseudogeneImmunogenetics 28: 406–411, 1988
Ways, J. P., Coppin, H. L., and Parham, P.: The complete primary structure of HLA-Bw 58.J Biol Chem 260: 11924–11933, 1985
Wolfe, J. H., Haskins, M. E., and Zmijewski, C. M.: Mixed lymphocyte reactivity in cats.Transplantation 37: 509–513, 1984
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M27192.
Rights and permissions
About this article
Cite this article
van Hoof, M., de Geus, J.P., Roos, M. et al. The primary structure of a feline class I gene: Striking similarity toHLA-A . Immunogenetics 30, 330–334 (1989). https://doi.org/10.1007/BF02425272
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02425272