Abstract
An HLA-B44 cDNA clone was identified in a cDNA library constructed from an HLA-B44 homozygous cell line. The DNA sequence was determined and was found to contain the complete coding sequence but for (probably) the three N-terminal codons. Comparisons of the derived amino acid sequence with other HLA-A and -B locus amino acid sequences revealed four HLA-B44-specific substitutions including a new polymorphic site. Regions of strong sequence conservation for HLA-B-locus products were found at the nucleotide and amino acid levels.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aviv, H. and Leder, P.: Purification of biologically active globin messenger RNA by chromatography on ofgothymidylic acid — cellulose. Proc. Natl. Acad. Sci. U.S.A. 69: 1408–1412, 1972
Biro, P. A., Pan, J., Sood, A. K., Kole, R., Reddy, V. B., and Weissman, S. M.: The major histocompatibiliy complex. Cold Spring Harbour Symp. Quant. Biol. 47: 1079–1086, 1983
Bodmer, W. F., Albert, E., Bodmer, J. G., Dausset, J., Kissmeyer-Nielsen, F., Mayr, W., Payne, R., van Rood, J. J., Trnka, Z., and Walford, R. L.: Nomenclature for factors of the HLA system 1984. Immunogenetics 20: 593–601, 1984
Buell, G. N., Wickens, M. P., Payvar, F., and Schimke, R. T.: Synthesis of full length cDNAs from four partially purified oviduct mRNAs. J. Biol. Chem. 253: 2471–2482, 1977
Cowan, E. P., Jordan, B. R., and Coligan, J. E.: Molecular cloning and DNA sequence analysis of genes encoding cytotoxic T lymphocyte-defined HLA-A3 subtypes: The EL subtype. J. Immunol. 135: 2835–2841, 1985
Efstratiadis, A. and Villa-Komaroff, L.: Cloning of double stranded DNA. In J. K. Stelow and A. Hollaender (eds.): Genetic Engineering, pp. 1–15, Plenum Press, New York, 1979
Ezquerra, A., Bragado, R., Vega, M. A., Strominger, J. L., Woody, J. N., and Lopez de Castro, J. A.: Primary structure of papainesolubilized human histocompatibility antigen HLA-B27. Biochemistry 24: 1733–1741, 1985
Hahn, A. W. A., Schendel, D. J., Hansen, P. W., and Ploegh, H. L.: Biochemical and cellular identification of HLA-Bw44 subtypes. Hum. Immunol. 11: 69–76, 1984
Koller, B. H. and Orr, H. T.: Cloning and complete sequence of an HLA-A2 gene: Analysis of two HLA-A alleles at the nucleotide level. J. Immunol. 134: 2727–2733, 1985
Koller, B. H., Sidwell, B., DeMars, R., and Orr, H. T.: Isolation of HLA locus specific DNA probes from the 3 untranslated region. Proc. Natl. Acad. Sci. U.S.A. 81: 5175–5178, 1984
Lopez de Castro, J. A., Strominger, J. L., Strong, D. M., and Orr, H. T.: Structure of crossreactive human histocompatibility antigens HLAA28 and HLA-A2: Possible implications for the generation of the HLA polymorphism. Proc. Natl. Acad. Sci. U.S.A. 79: 3813–3817, 1982
Lopez de Castro, J., Bragado, R., Strong, D. H., and Strominger, J. L.: Primary structure of papaine-solubilized human histocompatibility antigen HLA-B27. Biochemistry 22: 3961–3969, 1983
Lopez de Castro, J., Barbosa, J. A., Krangel, M. S., Biro, P. A., and Strominger, J. L.: Structural analysis of the functional site of class I HLA antigens. Immunol. Rev. 85: 148–168, 1985
Malissen, M., Malissen, B., and Jordan, B. R.: Exon/intronorganization and complete nucleotide sequence of an HLA gene. Proc. Natl. Acad. Sci. U.S.A. 79, 893–897, 1982
Maxam, A. M. and Gilbert, W.: Sequencing end labeled DNA with base specific chemical cleavages. Methods Enzymol. 65: 499–534, 1980
Michelson, A. M. and Orkin, S. H.: Characterization of the homopolymer tailing reaction catalyzed by terminal deoxynucleotidyl transferase (implications for the cloning of cDNA). J. Biol. Chem. 257: 14773–14782, 1982
N'Guyen, C., Sodoyer, R., Trucy, J., Strachan, T., and Jordan, B. R.: The HLA-Aw24 gene: Sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes. Immunogenetics 21: 479–489, 1985
Orr, H. T. and DeMars, R.: Class I-like HLA genes map telomeric to the HLA-2 locus in human cells. Nature 302: 534–536, 1983
Parham, P.: Monoclonal antibodies against two separate alloantigenic sites of HLA-B40. Immunogenetics 13: 509–527, 1981
Parham, P.: Antigenic determinants of the HLAB7 molecule; BW6- and B7-specific determinants are spatially separate. Immunogenetics 18: 1–16, 1983
Parham, P., Antonelli, P., Herzenberg, L. A., Kipps, T. J., Fuller, A., and Ward, F. E.: Further studies on the epitopes of HLA-B7 defined by murine monoclonal antibodies. Hum. Immunol. 15: 44–67, 1986
Ploegh, H. L., Orr, H.T., and Strominger, J. L.: Major histocompatibility antigens: The human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules. Cell 24: 287–299, 1981
Ruether, U. and Mueller-Hill, B.: Easy identification of cDNA clones. EMBO J. 2: 1791–1794, 1983
Sanger, F., Nicklen, S., and Coulsen, A. R.: DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74: 5463–5467, 1977
Seemann, G. H. A., Rein, R., Brown, C. S., and Ploegh, H. L.: Gene conversion like mechanisms generate polymorphism in human class I genes. EMBO J. 5: 547–552, 1986
Sodoyer, R., Damotte, M., Delovitch, T. L., Trucy, J., Jordan, B. R., and Strachan, T.: Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3). EMBO J. 3: 879–885, 1984
Sood, A. K., Pereira, D., and Weissman, S. M.: Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc. Natl. Acad. Sci. U.S.A. 78: 616–620, 1981
Steinmetz, M. and Hood, L.: Genes of the major histocompatibility complex in mouse and man. Science 222: 727–733, 1983
Strachan, T., Sodoyer, R., Damotte, M., and Jordan, B. R.: Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: Implications for the evolution of HLA genes. EMBO J. 3: 887–894, 1984
Strominger, J. L.: Human histocompatibility antigens: Genes and proteins. In B. Pernis and H. J. Vogel (eds.): Cell Biology of the Major Histocompatibility Complex, pp. 17–24, Academic Press, Inc., London, 1986
van Rood, J. J., de Vries, R. R. P., and Bradley, B. A.: Genetics and biology of the HLA system. In M. E. Dorf (ed.): The Role of the Major Histocompatibility Complex in Immunobiology, pp. 59–114, 1981
Ways, J. P., Coppin, H. L., and Parham, P.: The complete primary structure of HLA-Bw58. J. Biol. Chem. 260: 11924–11933, 1985
Wickens, M. P., Buell, G. N., and Schimke, R. T.: Synthesis of doublestranded DNA complementary to lysozyme — ovomucoid and ovalbumin — mRNAs. J. Biol. Chem. 253: 2483–2495, 1977
Yanisch-Perron, C., Vierra, J., Messing, J.: Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pU19 vectors. Gene 33: 103–119, 1985
Zeff, R. A., Geier, S. S., Gopas, J., Geliebter, J., Schulze, D. H., Pease, L. R.,Pfaffenbach, G. M., Pontarotti, P., Mashimo, H., McGovern, D. A., and Nathenson, S. G.: Mutants of the murine major histocompatibility complex: Structural analysis of in vivo and in vitro H-2Kb variants. In B. Pernis and H. J. Vogel (eds.): Cell Biology of the Major Histocompatibility Complex, pp. 41–49, Academic Press, Inc., London. 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kottmann, A.H., Seemann, G.H.A., Guessow, H.D. et al. DNA sequence of the coding region of the HLA-B44 gene. Immunogenetics 23, 396–400 (1986). https://doi.org/10.1007/BF00372673
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00372673