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Sequence of histidyl tRNA, present as a chloroplast insert in mtDNA ofZea mays

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Abstract

Unfractionated tRNA, isolated from maize mitochondria, has been specifically labeled at the -CCA end and used to recover a tRNA gene-bearing fragment from a clone bank of maize mitochondrial DNA. This gene has been mapped, sequenced and found to carry the anticodon for histidine. The sequence of the gene and that of bases in its near vicinity are identical to maize chloroplast tRNAHis, although sequences more distant on the fragment are not homologous with cpDNA. The junction of the cpDNA insert has been sequenced.

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Author notes

  1. Keith P. Iams

    Present address: ILRAD, P.O. Box 30709, Nairobi, Kenya

Authors and Affiliations

  1. Department of Biology, Indiana University, 47405, Bloomington, IN, USA

    Joyce E. Heckman

  2. Department of Chemistry, Indiana University, 47405, Bloomington, IN, USA

    John H. Sinclair

Authors
  1. Keith P. Iams
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  2. Joyce E. Heckman
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  3. John H. Sinclair
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Iams, K.P., Heckman, J.E. & Sinclair, J.H. Sequence of histidyl tRNA, present as a chloroplast insert in mtDNA ofZea mays . Plant Mol Biol 4, 225–232 (1985). https://doi.org/10.1007/BF02418240

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  • DOI: https://doi.org/10.1007/BF02418240

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