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Effect of diphosphonates on glycogen content of rabbit ear cartilage cells in culture

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Summary

The diphosphonates dichloromethanediphosphonate (Cl2MDP) and to a minor degree 1-hydroxyethane-1,1-diphosphonate (EDHP) increased the glycogen content in cultured cartilage cells of rabbit ear. This effect was not due to different concentrations of glucose in the medium.

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References

  1. Fleisch, H., Russell, R.G.G., Francis, M.D.: Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo, Science165:1262–1264, 1969

    CAS  PubMed  Google Scholar 

  2. Fleisch, H.A., Russell, R.G.G., Bisaz, S., Mühlbauer, R.C., Williams, D.A.: The inhibitory effect of phosphonates on the formation of calcium phosphate crystals in vitro and on aortic and kidney calcification in vivo, Eur. J. Clin. Invest.1:12–18, 1970

    Article  CAS  PubMed  Google Scholar 

  3. Francis M.D., Russell, R.G.G., Fleisch, H.: Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo, Science165:1264–1266, 1969

    CAS  PubMed  Google Scholar 

  4. Russell, R.G.G., Mühlbauer, R.C., Bisaz, S., Williams, D.A., Fleisch, H.: The influence of pyrophosphate, condensed phosphates, phosphonates and other phosphate compounds on the dissolution of hydroxyapatite in vitro and on bone resorption induced by parathyroid hormone in tissue culture and in thyroparathyroidectomised rats, Calcif. Tissue Res.6:183–196, 1970

    Article  CAS  PubMed  Google Scholar 

  5. Guilland, D.F., Fleisch, H.: The effect of in vivo treatment with EHDP and/or 1,25-DHCC on calcium uptake and release in isolated kidney mitochondria, Biochim. Biophys. Res. Commun.61:906–911, 1974

    Article  CAS  Google Scholar 

  6. Guilland, D.F., Sallis, J.D., Fleisch, H.: The effect of two diphosphonates on the handling of calcium by rat kidney mitochondria in vitro, Calcif. Tissue Res.15:303–314, 1974

    Article  CAS  PubMed  Google Scholar 

  7. Felix, R., Fleisch, H.: Increase in alkaline phosphatase activity in calvaria cells cultured with diphosphonates, Biochem. J.183:73–81, 1979

    CAS  PubMed  Google Scholar 

  8. Fast, D.K., Felix, R., Dowse, C., Neuman, W.F., Fleisch, H.: The effects of diphosphonates on the growth and glycolysis of connective-tissue cells in culture, Biochem. J.172:97–107, 1978

    CAS  PubMed  Google Scholar 

  9. Eagle, H.: Amino acid metabolism in mammalian cell cultures, Science130:432–437, 1959

    CAS  PubMed  Google Scholar 

  10. Bergmeyer, H.U., Bernt, E., Schmidt, F., Stork, H.: D-glucose: determination with hexokinase and glucose 6-phosphate dehydrogenase. In H.U. Bergmeyer (ed.): Methods of Enzymatic Analysis, Vol. 3, 2nd Ed., pp. 1196–1201. Verlag Chemie Weinheim, Academic Press, New York, 1974

    Google Scholar 

  11. Nahorski, S.R., Rogers, K.J.: An enzymic fluorometric micro method for determination of glycogen, Anal. Biochem.49:492–497, 1972

    Article  CAS  PubMed  Google Scholar 

  12. Larner, J., Villar-Palasi, C.: Glycogen synthase and its control. Curr. Top. Cell. Regul.3:195–236, 1971

    CAS  Google Scholar 

  13. Porter, C.A.: Method for increasing the sucrose content of growing plants, US Patent 3,826,641, July 30, 1974

  14. Amir, J., Wright, R.D., Cherry, J.H.: Chemical control of sucrose conversion to polysaccharides in sweet corn after harvest, J. Agr. Food Chem.19:954–957, 1971

    Article  CAS  Google Scholar 

  15. Guenther, H.L., Guenther, H.E., Fleisch, H.: Effect of ethane-1-hydroxy-1,1-diphosphonate and dichloromethane-diphosphonate on rabbit articular chondrocytes in culture, Biochem. J.184:203–214, 1979

    CAS  PubMed  Google Scholar 

  16. Guenther, H.L., Guenther, H.E., Fleisch, H.: The effect of dichloromethane diphosphonic acid (Cl2MDP) on the biosynthesis of rat bone and cartilage collagen in vivo and in vitro, Upsala J. Med. Sci.82:132, 1977

    Google Scholar 

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Felix, R., Fast, D.K., Sallis, J.D. et al. Effect of diphosphonates on glycogen content of rabbit ear cartilage cells in culture. Calcif Tissue Int 30, 163–166 (1980). https://doi.org/10.1007/BF02408621

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  • DOI: https://doi.org/10.1007/BF02408621

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