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The effect of glucose-6-phosphate isomerase genotype onin vitro specific activity andin vivo flux inMytilus edulis

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Abstract

Four samples of the musselMytilus edulis were taken between 1984 and 1987 from Stony Brook, New York, and used to study the glucose-6-phosphate isomerase (GPI) polymorphism in this species.In vitro specific activity andin vivo flux measured in the same animals were found to be significantly correlated. A significant effect of GPI genotype on flux was observed in one of the samples; overall, significant evidence of effect of genotype on enzyme activity was also obtained. GPI activities of common genotypes tend to deviate less from the population mean than those of rare (frequency less than 5%) genotypes. This suggests the possibility that rare GPI genotypes are rare as a consequence of having biochemical properties that deviate from an optimum level and, therefore, having a lower fitness. In support of this hypothesis, we found in one of our samples that shell length is a concave function of GPI activity with an intermediate optimum activity level.

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Author notes

  1. Walter J. Diehl III

    Present address: Department of Biological Sciences, Mississippi State University, P.O. Drawer GY, 39762, Mississippi State, Mississippi

  2. Elaine B. Winshell

    Present address: Ramapo College of New Jersey, School of Theoretical and Applied Science, 505 Ramapo Valley Road, 07430, Mahwah, New Jersey

  3. Mauro Santos

    Present address: Departmento de Genética, Facultad de Ciencias, Universidad Autónoma de Barcelona, Barcelona, Spain

Authors and Affiliations

  1. Department of Ecology and Evolution, State University of New York at Stony Brook, 11794, Stony Brook, New York

    Pedro J. N. Silva, Richard K. Koehn, Walter J. Diehl III, Robin P. Ertl, Elaine B. Winshell & Mauro Santos

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  1. Pedro J. N. Silva
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  2. Richard K. Koehn
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  3. Walter J. Diehl III
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  4. Robin P. Ertl
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Additional information

The financial support provided to P.J.N.S. by the Luso-American Educational Commission (Fulbright Program), the Instituto Nacional de Investigacao Científica (Portugal), and the Faculdade de Ciências da Universidade de Lisboa during several stages of this research is gratefully acknowledged. Financial support from the Ministerio de Educatión y Ciencia (Spain) in the form of a postdoctoral Fulbright/MEC fellowship to M.S. is also gratefully acknowledged. Research was supported by National Science Foundation Grant BSR-8415060 to R.K.K. This is contribution No. 736 from the Program in Ecology and Evolution, State University of New York at Stony Brook.

On leave from Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande C2, Lisboa, Portugal.

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Silva, P.J.N., Koehn, R.K., Diehl, W.J. et al. The effect of glucose-6-phosphate isomerase genotype onin vitro specific activity andin vivo flux inMytilus edulis . Biochem Genet 27, 451–467 (1989). https://doi.org/10.1007/BF02399674

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  • DOI: https://doi.org/10.1007/BF02399674

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