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Observation of transferrin hydration using Mössbauer spectroscopy

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Abstract

Pig transferrin was enriched with57Fe isotopes and its Mössbauer spectra were recorded using a57Co source diffused into a rhodium matrix. The transferrin was examined in freeze-dried form, in solution and in a partially hydrated state. The spectra in the first two cases consist of two peaks, which were interpreted as quadrupole doublets. Hydrated transferrin exhibits a more complex spectrum which can fairly well be approximated by summing the spectra of dry and dissolved samples with nearly equal contributions. From this fact, it may be concluded that both the chemical shift and quadrupole splitting either for dissolved or for hydrated specimens are determined by the same amount of water molecules situated in the neighbourhood vicinity of the iron binding sites.

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Author notes

  1. F. Franek

    Present address: Institute of Molecular Genetics, Prague, Czechoslovakia

Authors and Affiliations

  1. Central Research Institute for Physics, Budapest, Hungary

    L. Cser, F. Franek, I. A. Gladkih & D. Horváth

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  1. L. Cser
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  2. F. Franek
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  3. I. A. Gladkih
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  4. D. Horváth
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Cser, L., Franek, F., Gladkih, I.A. et al. Observation of transferrin hydration using Mössbauer spectroscopy. Hyperfine Interact 36, 175–182 (1987). https://doi.org/10.1007/BF02395351

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  • DOI: https://doi.org/10.1007/BF02395351

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