Bioprocess Engineering

, Volume 15, Issue 5, pp 253–256 | Cite as

Continuous extraction of a recombinant cutinase from Escherichia coli disrupted cells with reversed micelles using a perforated rotating disc contactor

  • M. G. Carneiro-da-Cunha
  • M. R. Aires-Barros
  • J. M. S. Cabral
  • E. B. Tambourgi
Originals

Abstract

This work reports on the continuous extraction of a recombinant cutinase with reversed micelles using a perforated rotating disc contactor. Intracellular cutinase was directly extracted at 20°C with 100 mM AOT in isooctane from complex biological media of Escherichia coli disrupted cells. The optimal conditions for the direct extraction of the enzyme from media containing cell debris led to an extraction yield of 54.4%.

Keywords

Isooctane Cabral Lipolytic Enzyme Micellar Phase Fusarium Solani 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. G. Carneiro-da-Cunha
    • 1
  • M. R. Aires-Barros
    • 1
  • J. M. S. Cabral
    • 1
  • E. B. Tambourgi
    • 2
  1. 1.Laboratório de Engenharia Bioquímica, Instituto Superior TécnicoCentro de Engenharia Biológica e QuímicaLisboaPortugal
  2. 2.UNICAMP/FEQ/DESQCampinas-SPBrasil

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