Summary
The distribution of decorin and biglycan was investigated at the light and electron microscopical level in adult human articular cartilage. In general, the amount of decorin and biglycan was found to decrease with the depth of the layer of the cartilage. Decorin was found in the interterritorial matrix where most of the collagen is located. This fits in well with the assumption that decorin may modulate collagen metabolism. Biglycan was found next to the chondrocytes in the pericellular matrix and is assumed to be responsible for cellular activities. At the ultrastructural level, decorin was localized in the interterritorial matrix and in vesicles in chondrocytes. Biglycan was found, usually though not exclusively in the pericellular matrix. Both small proteoglycans were detected close to and on the collagen fibres and also associated with the more globular structures of the matrix between the fibrils. A double-staining approach revealed that the two molecules could be located along the same collagen fibril. However, staining for biglycan and decorin was not observed simultaneously within the same region of the fibre.
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References
Bianco,P.,Fisher,L. W.,Young,M. F.,Termine,J. D. &Gehron Robey,P. (1990) Expression and localization of the two small proteoglycans biglycan and decorin in developing human skeletal and non-skeletal tissues.J. Histochem. Cytochem. 38, 1549–63.
Bosse,A.,Schwarz,K.,Vollmer,E. &Kresse,H. (1993) Divergent and co-localization of the two small proteoglycans decorin and proteoglycan-100 in human skeletal tissue and tumors.J. Histochem. Cytochem. 41, 13–9.
DeMey,J.,Moermans,N.,Geuens,G.,Nydens,R. &DeBrabander,M. (1981) High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method.Cell Biol. Int. Rep. 5, 889–99.
Eggli,P. S.,Hunziker,E. B. &Schenk,R. K. (1988) Quantification of structural features characterizing weight- and lessweight-bearing regions in articular cartilage: a sterological analysis of medial femoral chondyles in young adult rabbits.Anat. Rec. 222, 217–27.
Faulk,W. P. &Taylor,G. M. (1971) An immunocolloid method for the electron microscopy.Immunochem. 8, 1081–92.
Fisher,L. W. (1993) Biglycan. InGuidebook to the Extracellular Matrix and Adhesion Proteins (edited byKreisI. &ValeR.), p. 21. Oxford: Oxford University Press.
Fleischmajer,R.,Fisher,L. W.,MacDonald,E. D.,Jacobs,L.,Perlish,J. S. &Termine,J. D. (1991) Decorin interacts with fibrillar collagen of embryonic and adult human skin.J. Struct. Biol. 106, 82–90.
Frens,G. (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions.Nature Phys. Sci. 241, 20–2.
Glössl,J.,Beck,M. &Kresse,H. (1984) Biosynthesis of proteodermatan sulfate in cultured human fibroblasts.J. Biol. Chem. 259, 514–23.
Heinegård,D. &Paulsson,M. (1984) Structure and metabolism of proteoglycans. InExtracellular Matrix Biochemistry (edited byPiez,K. A. &Reddi,A. H.), pp. 277–328. Amsterdam: Elsevier Science.
Herken,R.,Manshausen,B.,Fussek,M. &Bonatz,G. (1987) Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney.Histochemistry 87, 59–64.
Kähäri,V. M.,Larjava,H. &Uitto,J. (1991) Differential regulation of extracellular matrix proteoglycan (PG) gene expression.J. Biol. Chem. 266, 10 608–15.
Poole,A. R.,Webber,C.,Pidoux,I.,Choi,H. &Rosenberg,L. C. (1986) Localization of a dermatan sulfate proteoglycan (DS-PGII) in cartilage and the presence of an immunologically related species in other tissues.J. Histochem. Cytochem. 34, 619–25.
Poole,C. A.,Flint,M. H. &Beaumont,B. W. (1987) Chondrones in cartilage. Ultrastructural analysis of the pericellular microenvironment in adult human articular cartilage.J. Orthop. Res. 5, 509–22.
Rosenberg,L. C.,Choi,H. C.,Tang,L.,Johnson,T. L.,Pal,S.,Webber,C.,Reiner,A. &Poole,A. R. (1985) Isolation of dermatan sulfate proteoglycan from mature bovine articular cartilages.J. Biol. Chem. 260, 6304–13.
Schwarz,K.,Breuer,B. &Kresse,H. (1990) Biosynthesis and properties of a further member of the small chondroitin/ dermatan sulfate proteoglycan family.J. Biol Chem. 265, 22 023–8.
Slot,J. W. &Geuze,H. J. (1981) Sizing of protein A-colloidal gold probes for immunoelectron microscopy.J. Cell Biol. 90, 533–6.
Snow,A. D.,Henderson,M.,Nochlin,D.,Kresse,H. &Wight,T. N. (1992) Peripheral distribution of dermatan sulfate proteoglycans (decorin) in amloid-containing plaques and their presence in neurofibrillary tangles of Alzheimer's disease.J. Histochem. Cytochem. 40, 105–13.
Stanescu,V. (1990) The small proteoglycans of cartilage matrix.Sem. Arthr. Rheumat. 20, 51–64.
Vogel,K. G.,Paulsson,M. &Heinegård,D. (1984) Specific inhibition of type I and type II collagen fibrillogenesis by the small proteoglycan of tendon.Biochem. J. 223, 587–97.
Voss,B.,Gössl,J.,Cully,Z. &Kresse,H. (1986) Immunocytochemical investigation on the distribution of small chondroitin sulfate-dermatan sulfate proteoglycan in the human.J. Histochem. Cytochem. 34, 1013–9.
Winnemöller,M.,Schmidt,G. &Kresse,H. (1991) Influence of decorin on fibroblast adhesion to fibronectin.Eur. J. Cell Biol. 54, 10–7.
Witsch-Prehm,P.,Miehlke,R. &Kresse,H. (1992) Presence of small proteoglycan fragments in normal and arthritic human cartilage.Arthritis Rheum. 35, 1042–52.
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Miosge, N., Flachsbart, K., Goetz, W. et al. Light and electron microscopical immunohistochemical localization of the small proteoglycan core proteins decorin and biglycan in human knee joint cartilage. Histochem J 26, 939–945 (1994). https://doi.org/10.1007/BF02388571
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DOI: https://doi.org/10.1007/BF02388571