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Immunohistochemical localization of antioxidant enzymes during hamster kidney development

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Summary

Immunolocalization studies of hamster kidney development were performed using polyclonal antibodies to antioxidant enzymes, including antibodies to copper, zinc and manganese superoxide dismutases, catalase, glutathione peroxidase and glutathioneS-transferases and their subunits. Antibodies to extracellular matrix proteins were also studied to determine the temporal sequence between expression of immunoreactive protein for basement membrane proteins, which serve as markers of embryonic induction of nephron development, and antioxidant enzyme expression in kidney development. Immunoreactive proteins for antioxidant enzymes were not detectable in the developing kidney until after extracellular matrix proteins had been deposited. However, immunoreactive proteins for the antioxidant enzymes copper, zinc and manganese superoxide dismutases, catalase, and α class glutathioneS-transferase Ya subunit were detected in renal tubules before birth. μ class glutathioneS-transferase subunits Yb1 and Yb2 stained transitional epithelium at high levels before birth. Our results indicate: (1) each type of kidney cell has a unique antioxidant enzyme profile, (2) antioxidant enzymes are expressed in different types of cell at different times during development, but antioxidant enzyme immunoreactive protein was not present until after immunoreactive proteins for extracellular matrix molecules were detected, and (3) certain antioxidant enzymes are present before birth, indicating that high oxygen tension present at birth is not crucial for induction of immunoreactive protein.

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Authors and Affiliations

  1. Pathology Service, William S. Middleton Memorial Veterans Hospital, 2500 Overlook Terrace, 53705, Madison, WI, USA

    Terry D. Oberley & Joan M. Sempf

  2. Department of Pathology, University of Wisconsin Medical School, 53705, Madison, WI

    Terry D. Oberley

  3. Radiation Research Laboratory, University of Iowa College of Medicine, 52242, Iowa City, IA, USA

    Larry W. Oberley

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  1. Terry D. Oberley
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  2. Joan M. Sempf
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  3. Larry W. Oberley
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Oberley, T.D., Sempf, J.M. & Oberley, L.W. Immunohistochemical localization of antioxidant enzymes during hamster kidney development. Histochem J 27, 575–586 (1995). https://doi.org/10.1007/BF02388455

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  • DOI: https://doi.org/10.1007/BF02388455

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