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Development of an isolated, pulsatile blood-perfused rat lung model for evaluating the preserved lung functions

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Abstract

The purpose of this study was to develop an isolated, pulsatile blood-perfused rat lung model that allows us to evaluate the preserved lung functions. Lungs isolated from Sprague-Dawley rats, were perfused with venous whole blood by either a pulsatile or constant flow. The effuent was continuously deoxygenated with a 95% N2/5% CO2 gas mixture. Airway resistance, lung compliance, elastic work, flow resistive work, pulmonary vascular resistance, and blood gas analysis were assessed. Pressor responses toN G-monomethyl-l-arginine (l-NMMA) were compared between pulsatile and constant blood flow. At a flow of 0.1 ml/g body weight/min, pulsatile perfusion allowed for stable perfusion at least for 2h (mean 162.5±15.1 min) with stable aerodynamic and hemodynamic variables including blood gas tensions, whereas constant perfusion resulted in immediate lung failure. Whenl-NMMA was added to the perfusate, the mean pulmonary artery pressure did not show any change in the constant flow (6.0±2.6% increase), but did show a significant increase in the pulsatile flow (45±11% increase). Pulsatile blood flow reduced the pulmonary vascular resistance relative to the constant flow and allowed for a 2-h perfusion period to evaluate the lung function. The vasorelaxant mechanism in the pulsatile perfusion is related in part to the endothelial-dependent relaxation observed in the nitric oxide pathway.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Hokkaido University Hospital, N-14, W-5, Kita-ku, 060, Sapporo, Japan

    Shigeyuki Sasaki & Keishu Yasuda

  2. Division of Cardiothoracic Surgery, New England Deaconess Hospital, Harvard Medical School, Boston, MA, USA

    James D. McCully & Joseph LoCicero III

Authors
  1. Shigeyuki Sasaki
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  2. Keishu Yasuda
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  3. James D. McCully
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  4. Joseph LoCicero III
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Sasaki, S., Yasuda, K., McCully, J.D. et al. Development of an isolated, pulsatile blood-perfused rat lung model for evaluating the preserved lung functions. Surg Today 27, 1154–1159 (1997). https://doi.org/10.1007/BF02385685

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  • DOI: https://doi.org/10.1007/BF02385685

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