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Factors determining mineral deposition rate, mineral composition and mineral density distribution in the recalcification of decalcified rat dentin

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Abstract

Rat dentin, fixed with aldehyde or non-fixed, was decalcified in EDTA solution or acetic acid solution. The decalcified sections were recalcified with calcifying solution, and mineral density distribution and mineral composition of the recalcified dentin, and mineral deposition rate were examined.

Mineral deposition rate was faster on the fixed dentin than on the unfixed dentin. Mineral composition changed along with the progress of calcification. The Ca/P ratio of mineral depositing on the fixed section at the initial stage of recalcification was unusually high for the ratio of calcium phosphate, suggesting calcium binding to the matrix. On the non-fixed sections, however, no such high Ca/P ratio was noted, indicating deposition of calcium phosphate from the beginning. These results suggested the acceleration of the mineral deposition rate by the binding of calcium to the matrix in the fixed sections. While the picture of mineral density distribution also changed along with the progress of calcification, the picture in both fixed and nonfixed sections closely resembled that in vivo at the moderately calcified stage. This suggested that mineral density distribution is independent of calcium binding to the matrix at the initial stage. The role of CO3 in the calcifying solution is also discussed.

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Authors and Affiliations

  1. Department of Experimental Pharmacology Division of Functional Disorder Research Medical Research Institute, Tokyo Medical and Dental University, Japan

    Toshiko Ogata

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  1. Toshiko Ogata
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Ogata, T. Factors determining mineral deposition rate, mineral composition and mineral density distribution in the recalcification of decalcified rat dentin. J Bone Miner Metab 10, 8–17 (1992). https://doi.org/10.1007/BF02383456

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