Abstract
The characteristics of the transport system for inorganic phosphate (Pi) have been investigated in chicken osteoclasts. Pi transport in these cells was mainly Nadependent and also energy-dependent, suggesting the existence of Na-Pi cotransport system in these cells. While the time course of Pi transport in chicken osteoclasts was comparable to that in chicken mononuclear cells, the precursors of the osteoclasts, Pi transport was stimulated in a time- and dose-dependent fashion, when the osteoclasts were exposed to bone particles. The stimulation was not due tode novo synthesis of Pi carrier, since the stimulatory effect was very rapid with the maximal effect observed at 1 h and could not be blocked by 50 µM cycloheximide. On the other hand, inhibition of osteoclast attachment to bone particles by adding an excess of Arg-Gly-Asp-Ser peptide blocked the stimulation. In summary, stimulation of Pi transport in chicken osteoclasts was induced by bone particles. Attachment of the osteoclasts to bone particles with integrin receptors appeared to be crucial to this stimulation.
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References
Caverzasio J, Selz T, Bonjour JP: Characteristics of phosphate transport in osteoblastlike cells. Calcif Tissue Int 43: 83–87, 1988
Selz T, Caverzasio J, Bonjour JP: Regulation of Na-dependent Pi transport by parathyroid hormone in osteoblast-like cells. Am J Physiol 256: E93-E100, 1989.
Kunkler KJ, Everett LM, Breedlove DK et al.: Insulin stimulates sodiumdependent phosphate transport by osteoblast-like cells. Am J Physiol 260: E751-E755, 1991.
Alvarez JI, Teitelbaum SL, Blair HC et al.: Generation of avian cells resembling osteoclasts from mononuclear phagocytes. Endocrinology 128: 2324–2335, 1991
Miyauchi A, Hruska KA, Greenfield EM et al.: Osteoclast cytosolic calcium, regulated by voltagegated calcium channels and extracellular calcium, controls podosome assembly and bone resorption. J Cell Biol 111: 2543–2552, 1990
Miyauchi A, Alvarez J, Greenfield EM et al.: Recognition of osteopontin and related peptides by anανβ 3 integrin stimulates immediate cell signals in osteoclasts. J Biol Chem 266: 20369–20374, 1991
Ousler MJ, Bell LV, Clevinger B et al.: Identification of osteoclast-specific antibodies. J Cell Biol 100: 1590–1600, 1985
Davis J, Earwich J, Totty N et al.: The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol 109: 1817–1826, 1989
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254, 1976
Reinholt FP, Hultenby K, Oldberg A et al.: Osteopontin-a possible anchor of osteoclasts to bone. Proc Natl Acad Sci USA 87: 4473–4475, 1990
Kaufmann M, Muff R, Fischer JA: Effect of dexamethasone on parathyroid hormone (PTH) and PTH-related protein regulated phosphate uptake in opossum kidney cells. Endocrinology 128: 1819–1824, 1991
Nakai M, Fukase M, Yamaguchi T et al.: Human PTH-(3-34) inhibited the effects of human parathyroid hormone-related protein on phosphate uptake in a cultured renal cell line (OK cells). J Bone Miner Res 5: 995–1002, 1990
Malmström K, Murer H: Parathyroid hormone inhibits phosphate transport in OK cells but not in LLC-PK2 and JTC-12.P3 cells. Am J Physiol 251: C23-C31, 1986
Caverzasio J, Rizzoli R, Bonjour JP: Sodium-dependent phosphate transport inhibited by parathyroid hormone and cyclic AMP stimulation in an opossum kidney cell line. J Biol Chem 261: 3233–3237, 1986
Kinoshita Y, Fukase M, Miyauchi A et al.: Establishment of a parathyroid hormone-responsive phosphate transport systemin vitro using cultured renal cells. Endocrinology 119: 1954–1963, 1986
Werner A, Moore ML, Mantei N et al.: Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex. Proc Natl Acad Sci USA 88: 9608–9612, 1991
Murer H, Werner A, Reshkin S et al.: Cellular mechanisms in proximal tubular reabsorption of inorganic phosphate. Am J Physiol 260: C885-C899, 1991
Blair HC, Teitelbaum SL, Ghiselli R et al.: Osteoclastic bone resorption by a polarized vacuolar proton pump. Science 245: 855–857, 1989
Zambonin-Zallone A, Teti A, Grano M et al.: Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: Aβ 3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells. Exp Cell Res 182: 645–652, 1989
Horton MA: Osteoclast-specific antigens. ISI Atlas Sci Immunol 1: 35–43, 1988
Hruska KA, Rolnick F, Huskey M: Occupancy of the osteoclastανβ 3 integrin by osteopontin stimulates a novelsrc associated phosphatidylinositol 3 kinase (PI3 kinase) resulting in phosphatidylinositol trisphosphate (PIP3) formation. J Bone Miner Res 7: S106, 1992 (Abstract)
Rolnick F, Huskey M, Gupta A et al.: The signal generating complex of the occupied osteoclastανβ 3 integrin includessrc, phosphatidyl inositol 3 kinase (PI3 kinase) and phospholipase Cγ (PLCγ). J Bone Miner Res 7: S105, 1992 (Abstract)
Hortn MA, Taylor ML, Arnett TR et al.: Arg-Gly-Asp (RGD) peptides and the anti-vitronectinin receptor antibody 23C6 inhibit dentine resorption and cell spreading by osteoclasts. Exp Cell Res 195: 368–375, 1991
Van der Pluijm G, Mouthaan H, Papapoulos S, et al.: Inhibition of osteoclast precursor attachment and subsequent bone resorption by synthetic RGD-peptides. J Bone Miner Res 6: S146, 1991 (Abstract)
Yates AJ, Oreffo ROC, Mayor K et al.: Inhibition of bone resorption by inorganic phosphate is mediated by both reduced osteoclast formation and decreased activity of mature osteoclasts. J Bone Miner Res 6: 473–478, 1991
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Fujimori, A., Miyauchi, A., Fukase, M. et al. Exposure to bone particles stimulates phosphate transport in chicken osteoclasts: Implication of the integrin receptor. J Bone Miner Metab 12 (Suppl 2), 43–50 (1994). https://doi.org/10.1007/BF02383383
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DOI: https://doi.org/10.1007/BF02383383