Abstract
Quail bone fragments cultured in the medium containing 17β estradiol (E2) were examined morphologically. On the endosteal bone surface of bones cultured in serum-containing medium, preosteoblasts were observed and labeled by3H-thymidine after 24 h of culturing. After 48 h of culturing, medullary bones occasionally appeared along the endosteal surface, and their bone surfaces were covered with labeled osteoblasts. These osteoblasts were frequently found when added E2. On the endosteal bone surface of cultured bones in the serum-free medium alone, bone lining cells were observed and not labeled throughout the culture period. On the endosteal bone surface of bones cultured in the serum-free medium containing E2, labeled preosteoblasts were seen after 24 and 48 h of culturing. However, osteoblasts did not appear. These findings suggest that estrogen acts directly on the proliferation and differentiation of osteogenic cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Mueller, W.J., Schraer, R. and Schraer, H.: Calcium metabolism and skeletal dynamics of laying pullets. J. Nutr. 84, 20–26, 1964.
Candlish, J.K.: The formation of mineral and organic matrix of fowl cortical and medullary bone during shell calcification. Br. Poult. Sci. 12, 119–127, 1971.
Bloom, M.A., McLean, F.C. and Bloom, W.: Calcification and ossification. Anat. Rec. 83, 99–120, 1942.
Kusuhara, S. and Schraer, H.: Cytology and autoradiography of estrogen induced differentiation of avian endosteal cells. Calcif. Tissue Int. 34, 352–358, 1982.
Bowman, B.M. and Miller, S.C.: The proliferation and differentiation of the bone lining cells in estrogen induced osteogenesis. Bone. 7, 351–357, 1986.
Ohashi, T., Kusuhara, S. and Ishida, K.: Effects of oestrogen and anti-oestrogen on the cells of the endosteal surface of male Japanese quail. Br. Poult. Sci. 28, 727–732, 1987.
Ohashi, T., Kusuhara, S. and Ishida, K.: Estrogen target cells during the early stage of medullary bone osteogenesis: immunohistochemical detection of estrogen receptors in osteogenic cells of estrogen treated male Japanese quail. Calcif. Tissue Int. 49, 124–127, 1991.
Ohashi, T., Kusuhara, S. and Ishida, K.: Immunoelectron microscopic demonstration of estrogen receptors in osteogenic cells of Japanese quail. Histochemistry. 96, 41–44, 1991.
Berthois, Y., Katzenellenbogen J.A. and Katzenellebogen B.S.: Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen responsive cells in culture. Proc. Natl. Acad. Sci. USA. 83, 2496–2500, 1986.
Welshons, W.V., Wolf, M.F., Murphy, C.S. and Jordan V.J.: Estrogenic activity of phenol red. Molec. Cell Endocr. 57, 169–178, 1988.
Turner, R.T. and Schraer, H.: Estrogen-induced sequential changes in avian bone metabolism. Calcif. Tiss. Res. 24, 157–162, 1977.
Komm, B.S., Terpening, C.M., Benz, D.J., Graeme, K.A., Gallegos, A., Korc, M., Greene, G.L., O'Malley, B.W. and Haussler, M.R.: Estrogen binding, receptor mRNA, and biologic response in osteoblast like osteosarcoma cells. Science. 241, 81–84, 1988.
Eriksen, E.F., Colvard, D.S., Berg, N.J., Graham, M.L., Mann, K.G., Spelsberg, T.C. and Riggs, B.L.: Evidence of estrogen receptors in normal human osteoblast-like cells. Science. 241, 84–86, 1988.
Colvard, D., Spelsberg, T., Eriksen, E., Keeting, P. and Riggs, B.L.: Evidence of steroid receptors in human osteoblast like cells. Connect. Tiss. Res. 20, 33–40, 1989.
Ernst, M., Shmid, C. and Froesh, E.R.: Enhanced osteoblast proliferation and collagen gene expression by estradiol. Proc. Natl. Acad. Sci. USA. 85, 2307–2310, 1988.
Sömjen, D., Weisman, Y., Harell, A., Berger, E. and Kaye, A.M.: Direct and sex-specific stimulation by sex steroids of creatine kinase activity and DNA synthesis in rat bone. Proc. Natl. Acad. Sci. USA. 86, 3361–3365, 1989.
Ernst, M., Heath, J.K. and Rodan, G.A.: Estradiol effects on proliferation, messenger ribonucleic acid for collagen and insulin-like growth factor I, and parathyroid hormone-stimulated adenylate cyclase activity in osteoblastic cells from calvariae and long bones. Endocrinology. 125, 825–833, 1989.
Ree, A.H., Landmark, B.F., Eskild, W., Levy, F.O., Lahooti, H., Jahnsen, T., Aakvaag, A. and Hansson, V.: Autologous down-regulation of messenger ribonucleic acid and protein levels for estrogen receptors in MCF-7 cells: an inverse correlation to progesterone receptor levels. Endocrinology. 124, 2577–2583, 1989.
Author information
Authors and Affiliations
About this article
Cite this article
Ohashi, T., Kusuhara, S. Effects of estrogen on the proliferation and differentiation of osteogenic cells during the early stage of medullary bone formation in cultured quail bones. J Bone Miner Metab 9, 15–20 (1991). https://doi.org/10.1007/BF02374901
Issue Date:
DOI: https://doi.org/10.1007/BF02374901