Abstract
Two mutants defective in succinate utilization were isolated by NTG mutagenesis of the effective wild typeRhizobium meliloti strain S14. The mutants used carbon sources in a fashion similar to strain S14, but they were not able to grow on succinate, fumarate or malate. The mutants nodulated alfalfa plants but did not exhibit any nitrogenase activity. The mutants oxidized glucose and fructose, but were not able to oxidize organic acids. Cultured free-living bacteria of strain S14 appeared to have an inducible C4-dicarboxylic acid uptake system and a constitutive glucose uptake system. When S14 cells were grown on glucose in the presence of 5mM or more succinate or malate, the rate of glucose-dependent O2 consumption significantly decreased suggesting the presence of a catabolite repression like phenomenom.
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Contribution no. 301, Station de Recherches, Agriculture Canada.
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Lafreniere, C., Lafontaine, P., Marion, C. et al. Oxydation of substrates in organic acids utilization negative mutants and the wild typeRhizobium meliloti strain S'14 . Plant Soil 101, 73–78 (1987). https://doi.org/10.1007/BF02371033
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DOI: https://doi.org/10.1007/BF02371033