Résumé
L'étude des protéines des granules dePieris brassicae L. et des polyèdres nucléaires deBombyx mori L. par les techniques de filtration sur gel, d'électrophorèse et d'immunodiffusion révèle de nombreuses ressemblances. La comparaison des protéines des corps d'inclusion engendrés par lesBaculovirus des Lépidoptères contribue à montrer l'homogénéité de ce groupe.
Summary
It is generally admitted that the viruses of nuclear polyhedroses and granuloses of Lepidoptera form two distinct groups serologically.
A comparative study of proteins of nuclear polyhedral bodies ofBombyx mori and of granules ofPieris brassicae was carried by using techniques of gel filtration, electrophoresis and immunodiffusion. The characteristics of proteins of granules studied by gel chromatography and electrophoresis are closely related to those of proteins of polyhedral bodies.
In immunodiffusion, the extraction of proteins of polyhedral inclusion bodies by the carbonate reveals a common antigen (C antigen) which is recognized by homologue serums and anti-protein heterologue serums of inclusion bodies of seven otherBaculovirus. The extraction by the thioglycolate reveals, in addition to C antigens, specific antigens: T antigen for the granulosis ofPieris brassicae and B antigens for the nuclear polyhedrosis ofBombyx mori.
The comparison of proteins of inclusion bodies generated by granulosis and nuclear polyhedrosis viruses contributes to show the homogeneity of the LepidopterBaculovirus group.
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Avec la collaboration technique de LilianeCroizier.
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Croizier, G., Meynadier, G. Les protéines des corps d'inclusion desBaculovirus III. Étude comparée de la granulose dePieris brassicae et de la polyédrose deBombyx mori . Entomophaga 18, 431–437 (1973). https://doi.org/10.1007/BF02371020
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DOI: https://doi.org/10.1007/BF02371020