Skip to main content
SpringerLink
Log in

Les protéines des corps d’inclusion desBaculovirus 1. — Étude de leur solubilisation

  • Published:
Entomophaga Aims and scope Submit manuscript

Résumé

L’étude immunochimique des corps d’inclusion formés par lesBaculovirus de Lépidoptères est entreprise en vue de différencier ces viroses. Le travail relaté décrit l’influence de différents solutés sur l’extraction des protéines des granules de la granulose dePieris brassicae. L’intérêt de l’emploi d’agents réducteurs tels que l’acide thioglycolique ou le mercaptoéthanol pour la solubilisation des granules est mis en évidence.

Summary

The proteins of inclusion bodies of Baculoviruses (pathogens causing granulosis and nuclear polyhedrosis) are peculiar to insect viruses. The study of their immunochemical and biochemical characteristics should make it possible to establish an accurate differential diagnosis.

The present work is a preliminary study, the objective of which is to determine optimum conditions for the solubilization of inclusion bodies.

The following solutions are used for dissolving granules of the granulosis ofPieris brassicae:

  1. 1°

    CO3Na2 (0,06-0,25M)+ClNa 0,05M (Bergold’ process, 1963).

  2. 2°

    Tris 2 M.

  3. 3°

    Mixtures of tris and urea.

  4. 4°

    Sodium thioglycolate-bicarbonate (0,06–0,25 M), pH 10,5.

  5. 5°

    2-mercaptoethanol 1% in tris 1 M.

The proteins released are analysed by immunodiffusion and starch gel electrophoresis. Two different proteins are revealed: the T protein is extracted when reducing agents are added (thioglycolic acid, 2-mercaptoethanol) in alkaline solutions; the C protein is released in any case.

The use of reducing agents improves the classical process of solubilization of inclusion bodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Bibliographie

  • Aleksandrov, J. M. — 1965. Recherches des relations sérologiques entre la protéine des polyèdres et le virus de la polyédrose nucléaire de certains Lépidoptères. —Mikrobiol. Zh. Ukrajin,27, 80–84.

    CAS  Google Scholar 

  • Bergoin, M., Scalla, R., Duthoit, J. L. &Vago, C. — 1957. Mise en évidence deVagoiavirus en microscopie électronique après dissolution des corps d’inclusion. In: Insect Pathology and microbial control (P. A. van der Laan, ed.), 43–68,North-Holland Publ. Co., Amsterdam.

    Google Scholar 

  • Bergoin, M., Veyrunes, J. C. &Scalla, R. — 1970. Isolation and amino-acid composition of the inclusions ofMelolontha melolontha Poxvirus. —Virology,40 761–763.

    Article  CAS  PubMed  Google Scholar 

  • Bergold, G. H. — 1963. The nature of nuclear-polyhedrosis viruses. In: Insect Pathology (Steinhauss, E. A., ed.), vol. I, 413–456,Academic Press, N. Y.

    Google Scholar 

  • Cunningham, J. C. — 1968. Serological and morphological identification of some nuclear-polyhedrosis and granulosis viruses. —J. Invertebr. Path.,11, 132–141.

    Google Scholar 

  • Croizier, G. &Meynadier, G. — 1971. Mise en évidence et comparaison d’antigènes extraits des formes géantes de troisRickettsiella Philip. —Ann. Inst. Pasteur,121, 87–92.

    CAS  Google Scholar 

  • Gershenson, S. M. — 1959. Mutation of polyhedrosis viruses. —Doklady Akad. Nauk S.S.S.R.,128, 622–625.

    Google Scholar 

  • — — 1960. A study on a mutant strain of nuclear polyhedral virus of oak silkworm. —Problems Virol. U.R.S.S.,6, 720–725.

    Google Scholar 

  • Himeno, M., Sakai, F., Onodera, K., Nakai, H., Fukada, T. &Kawade, Y. — 1967. Formation of nuclear polyhedral and nuclear polyhedrosis virus of silkworm in Mammalian cells infected with viral DNA. —Virology,33, 507–512.

    Article  CAS  PubMed  Google Scholar 

  • Huger, A. — 1963. Granuloses of Insects; In: Insect Pathology, (Steinhauss E. A., ed.), vol. I, 531–575,Academic Press, N. Y.

    Google Scholar 

  • Kristjansson, F. K. — 1964. Genetic control of two prealbumins in pigs. —Genetics,48, 1059–1063.

    Google Scholar 

  • Krywienczyk, J. — 1962., Immunoelectrophoretic studies of inclusion body proteins. —J. Insect Path.,4, 185–191.

    Google Scholar 

  • Krywienczyk, J. &Bergold, G. H. — 1960 a. Serologic relationship between inclusion body protein of some Lepidoptera and Hymenoptera. —J. Immunol.,84, 404–408.

    CAS  PubMed  Google Scholar 

  • — — 1960 b. Serological relationship between viruses and inclusion body proteins from some Lepidoptera and Hymenoptera. —J. Insect Pathol.,2, 118–123.

    Google Scholar 

  • — — 1961. Serological studies of inclusion body proteins by agar diffusion technique. —J. Insect Path.,3, 15–28.

    CAS  Google Scholar 

  • Longworth, J. F., Robertson, J. S., Payne, C. C. — 1971. The purification and properties of the inclusion body-protein of the granulosis virus ofPieris brassicae. — IVth. Internat. Colloq. on Insect Pathology, Maryland, U.S.A., 1970, 115–126.

  • Ouchterlony, O. — 1958. Diffusion-in-gel method for immunological analysis. —Progr. Allergy,5, 1–78.

    CAS  Google Scholar 

  • Poulik, M. D. — 1967. Starch in gel electrophoresis in discontinous system of buffers. —Nature,180, 1477–1479.

    Google Scholar 

  • Stairs, G. R. — 1964. Selection of a strain of insect granulosis virus producing only cubic inclusion bodies. —Virology,24, 520–521.

    Article  CAS  PubMed  Google Scholar 

  • Tarasevich, L. M. — 1945. Determination of the isoelectric point of virus proteins by staining. —C.R. Acad. Sc. U.R.S.S.,47, 694.

    CAS  Google Scholar 

  • Wildy, P. — 1971. Classification and nomenclature of viruses. In: Monographs in Virology, vol. 5, (Melnick, J. L. ed.), 81 pages,Karger S., Basel.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Station de recherches Cytopathologiques, Institut national de la Recherche agronomique, 30, Saint-Christol, France

    G. Croizier & G. Meynadier

Authors
  1. G. Croizier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Meynadier
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Avec la collaboration technique de LilianeCroizier.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Croizier, G., Meynadier, G. Les protéines des corps d’inclusion desBaculovirus 1. — Étude de leur solubilisation. Entomophaga 17, 231–239 (1972). https://doi.org/10.1007/BF02371133

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02371133

Search

Navigation