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A density method for determining plasma and red blood cell volume

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Abstract

We employed a highly sensitive density measuring system to measure the decrease in blood density and plasma density due to the infusion of a saline bolus into the rabbit's circulation. Based on a vasculature model with the Fahraeus effect, we deduced a set of equations to calculate from the density decreases the red-blood-cell volume and plasma volume of the rabbit. A value of 0.82±0.02 was obtained forF cell, the ratio of the hematocrit calculated from the two volumes and the arterial hematocrit.

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References

  1. Altman, P.L.; Dittmer, D.S., Biology data book. 2nd ed. Bethesda, MD: FASEB; 1974.

    Google Scholar 

  2. Baker, C.H.; Remington, J.W. Role of the spleen in determining total body hematocrit. Am. J. Physiol. 198:906–910; 1960.

    CAS  PubMed  Google Scholar 

  3. Barbee, J.H.; Cokelet, G.R. The Fahraeus effect. Microvasc. Res. 3:6–16; 1971.

    CAS  PubMed  Google Scholar 

  4. Brace, R.A. Blood volume and its measurement in the chronically catheterized sheep fetus. Am. J. Physiol. 244:H487-H494; 1983.

    CAS  PubMed  Google Scholar 

  5. Chien, S.; Dellenback, R.J.; Usami, S.; Gregersen, M.I. Plasma trapping in hematocrit determinations, differences among animal species. Proc. Soc. Exp. Biol. & Med. 119:1155–1158; 1965.

    CAS  Google Scholar 

  6. Chien, S.; Simchon, S. Tissue hematocrit and hemorheology. In: Tomita, M.; Sawada, T.; Naritomi, H.; Heiss, W.D., eds. Cerebral hyperemia and ischemia: From the standpoint of cerebral volume. Amsterdam: Elsevier 1988:pp. 39–44.

    Google Scholar 

  7. Fung, Y.C. Biomechanics. NY: Springer-Verlag; 1981.

    Google Scholar 

  8. Hinghofer-Szalkay, H. Continuous blood densitometry: Fluid shifts after graded hemorrhage in animals. Am. J. Physiol. 250:H342-H350; 1986.

    CAS  PubMed  Google Scholar 

  9. Hinghofer-Szalkay, H.; Leopold, H.; Kenner, T.; Holzer, H. On the coefficient of thermal expansion of blood and the constituents. Biomed. Technik. 25:151–157; 1980.

    CAS  Google Scholar 

  10. Gamas, L.; Lee, J.S. A density indicator method to measure pulmonary blood flow. J. Appl. Physiol. 60:327–334; 1985.

    Google Scholar 

  11. Kenner, T. Physiological measurement in circulation research, A review on the biological application of a new method. Med. Prog. Technol. 9:67–74; 1982.

    CAS  PubMed  Google Scholar 

  12. LaForte, A.J.; Lee, L.P.; Lee, J.S. Circulatory responses to cyclic changes in blood volume in anesthetized rabbits. FASEB J. 4:A1085; 1990.

    Google Scholar 

  13. Lee, J.S. Microvascular hematocrit of the lung. In: Schmid-Schonbeim, G.W.; Wu, S.; Zweifach, B.W., eds. Frontiers in biomechanics. New York: Springer-Veralg; 1986:pp. 353–364.

    Google Scholar 

  14. Lee, J.S.; Lee, L.P. Ventilatory changes of pulmonary capillary blood volume assessed by arterial density. J. Appl. Physiol. 61:1724–1731; 1986.

    CAS  PubMed  Google Scholar 

  15. Lipowsky, H.H.; Firrell, J.C. Microvascular hemodynamics during systemic hemodilution and hemoconcentration. Am. J. Physiol. 250:H908-H922; 1986.

    CAS  PubMed  Google Scholar 

  16. Lipowsky, H.H.; Usami, S.; Chien, S.In vivo measurements of “apparent viscosity” and microvessel hematocrit in the mesentery of the cat. Microvasc. Res. 19:297–319; 1980.

    Article  CAS  PubMed  Google Scholar 

  17. Pries, A.R.; Gaehtgens, P. Dispersion of blood cell flow in microvascular networks. In: Lee, J.S.; Skalak, T.C., eds. Microvascular mechanics. New York: Springer-Verlag; 1989:pp. 50–64.

    Google Scholar 

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Lee, Js., Lee, Lp. A density method for determining plasma and red blood cell volume. Ann Biomed Eng 20, 195–204 (1992). https://doi.org/10.1007/BF02368520

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  • DOI: https://doi.org/10.1007/BF02368520

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